Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 1947685..1948280 Replicon chromosome
Accession NZ_CP077646
Organism Escherichia coli strain ST866

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KTN06_RS09360 Protein ID WP_000239579.1
Coordinates 1947685..1948035 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KTN06_RS09365 Protein ID WP_001223208.1
Coordinates 1948029..1948280 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN06_RS09340 (1943131) 1943131..1944153 - 1023 WP_062893890.1 ABC transporter permease -
KTN06_RS09345 (1944167) 1944167..1945669 - 1503 WP_062893891.1 sugar ABC transporter ATP-binding protein -
KTN06_RS09350 (1945809) 1945809..1946765 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KTN06_RS09355 (1947075) 1947075..1947605 + 531 WP_000055072.1 inorganic diphosphatase -
KTN06_RS09360 (1947685) 1947685..1948035 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KTN06_RS09365 (1948029) 1948029..1948280 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KTN06_RS09370 (1948493) 1948493..1948834 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KTN06_RS09375 (1948837) 1948837..1952616 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T207255 WP_000239579.1 NZ_CP077646:c1948035-1947685 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T207255 NZ_CP102947:2182600-2182702 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT207255 WP_001223208.1 NZ_CP077646:c1948280-1948029 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT207255 NZ_CP102947:c2182740-2182472 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References