Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1323568..1324405 Replicon chromosome
Accession NZ_CP077646
Organism Escherichia coli strain ST866

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag KTN06_RS06480 Protein ID WP_000227784.1
Coordinates 1323863..1324405 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag KTN06_RS06475 Protein ID WP_001297137.1
Coordinates 1323568..1323879 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN06_RS06450 (1318588) 1318588..1319535 + 948 WP_217609421.1 cytochrome o ubiquinol oxidase subunit II -
KTN06_RS06455 (1319557) 1319557..1321548 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KTN06_RS06460 (1321538) 1321538..1322152 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KTN06_RS06465 (1322152) 1322152..1322481 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KTN06_RS06470 (1322493) 1322493..1323383 + 891 WP_135301382.1 heme o synthase -
KTN06_RS06475 (1323568) 1323568..1323879 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
KTN06_RS06480 (1323863) 1323863..1324405 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
KTN06_RS06485 (1324461) 1324461..1325396 - 936 WP_001297127.1 tetratricopeptide repeat protein -
KTN06_RS06490 (1325804) 1325804..1327168 + 1365 WP_001000978.1 MFS transporter -
KTN06_RS06495 (1327296) 1327296..1327787 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KTN06_RS06500 (1327955) 1327955..1328866 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T207253 WP_000227784.1 NZ_CP077646:1323863-1324405 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T207253 NZ_CP102947:1910941-1911195 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT207253 WP_001297137.1 NZ_CP077646:1323568-1323879 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT207253 NZ_CP102947:1910693-1910944 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References