Detailed information of TA system TA207251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 230394..231032
• Replicon: chromosome
• Accession: NZ_CP077646
• Organism: Escherichia coli strain ST866

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KTN06_RS01095
• Protein ID: WP_000813794.1
• Coordinates: 230856..231032 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KTN06_RS01090
• Protein ID: WP_001270286.1
• Coordinates: 230394..230810 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTN06_RS01070 (225546)	225546..226487	-	942	WP_001251315.1	ABC transporter permease	-
KTN06_RS01075 (226488)	226488..227501	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KTN06_RS01080 (227519)	227519..228664	-	1146	WP_217609364.1	ABC transporter substrate-binding protein	-
KTN06_RS01085 (228909)	228909..230315	-	1407	WP_000760615.1	PLP-dependent aminotransferase family protein	-
KTN06_RS01090 (230394)	230394..230810	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KTN06_RS01095 (230856)	230856..231032	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KTN06_RS01100 (231254)	231254..231484	+	231	WP_000494244.1	YncJ family protein	-
KTN06_RS01105 (231576)	231576..233537	-	1962	WP_050867317.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KTN06_RS01110 (233610)	233610..234146	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KTN06_RS01115 (234238)	234238..235413	+	1176	WP_001236258.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T207251 WP_000813794.1 NZ_CP077646:c231032-230856 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T207251 NZ_CP102947:966300-966707 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT207251 WP_001270286.1 NZ_CP077646:c230810-230394 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT207251 NZ_CP102947:966053-966319 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6HPB