Detailed information of TA system TA207249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 548348..548978
• Replicon: chromosome
• Accession: NZ_CP077639
• Organism: Terribacillus sp. DMT04

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KS242_RS02990
• Protein ID: WP_077305197.1
• Coordinates: 548628..548978 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KS242_RS02985
• Protein ID: WP_217322964.1
• Coordinates: 548348..548623 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KS242_RS02965 (KS242_02965)	543921..544286	+	366	WP_217322960.1	holo-ACP synthase	-
KS242_RS02970 (KS242_02970)	544348..545859	+	1512	WP_217322961.1	NAD(P)H-hydrate dehydratase	-
KS242_RS02975 (KS242_02975)	545910..546926	+	1017	WP_217322962.1	outer membrane lipoprotein carrier protein LolA	-
KS242_RS02980 (KS242_02980)	547019..548191	+	1173	WP_217322963.1	alanine racemase	-
KS242_RS02985 (KS242_02985)	548348..548623	+	276	WP_217322964.1	antitoxin	Antitoxin
KS242_RS02990 (KS242_02990)	548628..548978	+	351	WP_077305197.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KS242_RS02995 (KS242_02995)	549187..550059	+	873	WP_217322965.1	RsbT co-antagonist protein RsbRA	-
KS242_RS03000 (KS242_03000)	550056..550412	+	357	WP_038558142.1	STAS domain-containing protein	-
KS242_RS03005 (KS242_03005)	550415..550816	+	402	WP_077305194.1	anti-sigma regulatory factor	-
KS242_RS03010 (KS242_03010)	550833..551843	+	1011	WP_217322966.1	PP2C family protein-serine/threonine phosphatase	-
KS242_RS03015 (KS242_03015)	551900..552229	+	330	WP_217322967.1	anti-sigma factor antagonist	-
KS242_RS03020 (KS242_03020)	552229..552708	+	480	WP_179106470.1	anti-sigma B factor RsbW	-
KS242_RS03025 (KS242_03025)	552680..553471	+	792	WP_217322968.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12997.07 Da
• Isoelectric Point: 5.8701

>T207249 WP_077305197.1 NZ_CP077639:548628-548978 [Terribacillus sp. DMT04]
MIVKRGEVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITQLDTQMMENINRALEISLGLTEF

Nucleotide

• Length: 351 bp

>T207249 NZ_CP102947:644381-644695 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10452.89 Da
• Isoelectric Point: 4.4118

>AT207249 WP_217322964.1 NZ_CP077639:548348-548623 [Terribacillus sp. DMT04]
MVVSEGLQEIVVKLPKKLLNEVDGLMQNNNDSLDHFFYQATKSYVQERKDIRETMQKGYMEMARINLNIASEAFQAEEEA
EVTLERLVSGV

Nucleotide

• Length: 276 bp

>AT207249 NZ_CP102947:644692-645108 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA