Detailed information of TA system TA207248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 4350192..4350723
• Replicon: chromosome
• Accession: NZ_CP077638
• Organism: Methylorubrum extorquens strain PA1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KQ926_RS20405
• Protein ID: WP_003605878.1
• Coordinates: 4350442..4350723 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C5B2A4
• Locus tag: KQ926_RS20400
• Protein ID: WP_003605879.1
• Coordinates: 4350192..4350455 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ926_RS20380 (KQ926_20205)	4346232..4346858	+	627	WP_042508980.1	bifunctional nicotinamidase/pyrazinamidase	-
KQ926_RS20385 (KQ926_20210)	4347029..4347886	-	858	WP_012255092.1	aminotransferase class IV	-
KQ926_RS20390 (KQ926_20215)	4347873..4348502	-	630	WP_012255093.1	aminodeoxychorismate/anthranilate synthase component II	-
KQ926_RS20395 (KQ926_20220)	4348499..4349899	-	1401	WP_042508981.1	aminodeoxychorismate synthase component I	-
KQ926_RS20400 (KQ926_20225)	4350192..4350455	+	264	WP_003605879.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KQ926_RS20405 (KQ926_20230)	4350442..4350723	+	282	WP_003605878.1	type II toxin-antitoxin system YafQ family toxin	Toxin
KQ926_RS20410 (KQ926_20235)	4350840..4352186	+	1347	WP_012255095.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KQ926_RS20415 (KQ926_20240)	4352283..4352468	+	186	WP_003605874.1	hypothetical protein	-
KQ926_RS20420 (KQ926_20245)	4352475..4352897	+	423	WP_012255096.1	DoxX family protein	-
KQ926_RS20425 (KQ926_20250)	4353010..4353177	+	168	WP_245268102.1	hypothetical protein	-
KQ926_RS20430 (KQ926_20255)	4353263..4354093	-	831	WP_012255097.1	nucleoside triphosphate pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10664.24 Da
• Isoelectric Point: 10.2159

>T207248 WP_003605878.1 NZ_CP077638:4350442-4350723 [Methylorubrum extorquens]
MRTIERTTQFKRDYKREMKGRHRASLQADLTAVLTELAADRPLAARLRDHALTGNWADHRDCHVKPDLVLIYRLAGTETL
QLVRLGSHAELGF

Nucleotide

• Length: 282 bp

>T207248 NZ_CP102947:c603065-602601 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9356.81 Da
• Isoelectric Point: 4.4230

>AT207248 WP_003605879.1 NZ_CP077638:4350192-4350455 [Methylorubrum extorquens]
MPTKSLIQARIDADTKEEATAVLKAMGLTVSDAVRMMLVRVAREKALPFEPLVPNETTIAAIREARSGNLPGFTSVEALM
ADLDADD

Nucleotide

• Length: 264 bp

>AT207248 NZ_CP102947:c603400-603065 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: C5B2A4