Detailed information of TA system TA207246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 388704..389286
• Replicon: chromosome
• Accession: NZ_CP077638
• Organism: Methylorubrum extorquens strain PA1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KQ926_RS01860
• Protein ID: WP_012252142.1
• Coordinates: 389011..389286 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: KQ926_RS01855
• Protein ID: WP_012252141.1
• Coordinates: 388704..389021 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ926_RS01840 (KQ926_01790)	383976..384950	-	975	WP_012252139.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
KQ926_RS01845 (KQ926_01795)	385110..387896	+	2787	WP_003600762.1	[protein-PII] uridylyltransferase	-
KQ926_RS01850 (KQ926_01800)	387988..388596	+	609	WP_012252140.1	nucleotide exchange factor GrpE	-
KQ926_RS01855 (KQ926_01805)	388704..389021	-	318	WP_012252141.1	BrnA antitoxin family protein	Antitoxin
KQ926_RS01860 (KQ926_01810)	389011..389286	-	276	WP_012252142.1	BrnT family toxin	Toxin
KQ926_RS01865 (KQ926_01815)	389378..390235	-	858	WP_012252143.1	prephenate dehydratase	-
KQ926_RS01870 (KQ926_01820)	390252..390995	-	744	WP_003600755.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
KQ926_RS01875 (KQ926_01825)	391303..392220	+	918	WP_012252144.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10017.51 Da
• Isoelectric Point: 7.0130

>T207246 WP_012252142.1 NZ_CP077638:c389286-389011 [Methylorubrum extorquens]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVAVAQPSAAGSARMKIIGEFDGQTVVAAIIAPLGREAISLISLRRA
SRSERRLYDAR

Nucleotide

• Length: 276 bp

>T207246 NZ_CP102947:187871-187978 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11616.97 Da
• Isoelectric Point: 4.6461

>AT207246 WP_012252141.1 NZ_CP077638:c389021-388704 [Methylorubrum extorquens]
MPADKPDRTLTTNSEEAGIQAGIATDPDNPEWTEADFQSAVPFEQAFPVLAEKLRRTRGPQSAPTKQLVSLRLDQDVLLR
FRATGPGWQSRMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT207246 NZ_CP102947:c187814-187757 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT