Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3985576..3986195 Replicon chromosome
Accession NZ_CP077429
Organism Klebsiella aerogenes strain NTT31XS

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag KSL85_RS19115 Protein ID WP_015367918.1
Coordinates 3985977..3986195 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag KSL85_RS19110 Protein ID WP_015367917.1
Coordinates 3985576..3985950 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KSL85_RS19100 (KSL85_19100) 3980735..3981934 + 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KSL85_RS19105 (KSL85_19105) 3981957..3985103 + 3147 WP_015367916.1 multidrug efflux RND transporter permease subunit AcrB -
KSL85_RS19110 (KSL85_19110) 3985576..3985950 + 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
KSL85_RS19115 (KSL85_19115) 3985977..3986195 + 219 WP_015367918.1 HHA domain-containing protein Toxin
KSL85_RS19120 (KSL85_19120) 3986327..3986890 + 564 WP_015704609.1 maltose O-acetyltransferase -
KSL85_RS19125 (KSL85_19125) 3987016..3987480 + 465 WP_015367920.1 YlaC family protein -
KSL85_RS19130 (KSL85_19130) 3987455..3988909 - 1455 WP_049033189.1 PLP-dependent aminotransferase family protein -
KSL85_RS19135 (KSL85_19135) 3989012..3989722 + 711 WP_217074390.1 GNAT family protein -
KSL85_RS19140 (KSL85_19140) 3989719..3989859 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KSL85_RS19145 (KSL85_19145) 3989862..3990122 - 261 WP_015367923.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T207242 WP_015367918.1 NZ_CP077429:3985977-3986195 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207242 NZ_CP102946:c4767455-4767090 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT207242 WP_015367917.1 NZ_CP077429:3985576-3985950 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207242 NZ_CP102946:c4767097-4766774 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCAGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References