Detailed information of TA system TA207185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 149906..150657
• Replicon: chromosome
• Accession: NZ_CP077421
• Organism: Pectobacterium polaris strain QK413-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KSL88_RS00610
• Protein ID: WP_039545981.1
• Coordinates: 150175..150657 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A7V8QCC3
• Locus tag: KSL88_RS00605
• Protein ID: WP_039521400.1
• Coordinates: 149906..150184 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSL88_RS00580 (KSL88_00580)	145410..146072	-	663	WP_224072916.1	aldolase	-
KSL88_RS00585 (KSL88_00585)	146069..147343	-	1275	WP_224072917.1	four-carbon acid sugar kinase family protein	-
KSL88_RS00590 (KSL88_00590)	147340..148254	-	915	WP_205556788.1	NAD(P)-dependent oxidoreductase	-
KSL88_RS00595 (KSL88_00595)	148593..149354	+	762	WP_224072918.1	DNA-binding transcriptional repressor YgbI	-
KSL88_RS00600 (KSL88_00600)	149367..149795	+	429	WP_224072919.1	hypothetical protein	-
KSL88_RS00605 (KSL88_00605)	149906..150184	+	279	WP_039521400.1	DUF1778 domain-containing protein	Antitoxin
KSL88_RS00610 (KSL88_00610)	150175..150657	+	483	WP_039545981.1	GNAT family N-acetyltransferase	Toxin
KSL88_RS00615 (KSL88_00615)	150790..152124	+	1335	WP_095700951.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KSL88_RS00620 (KSL88_00620)	152197..153084	+	888	WP_095700950.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KSL88_RS00625 (KSL88_00625)	153110..153955	+	846	WP_095995326.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KSL88_RS00630 (KSL88_00630)	153981..155054	+	1074	WP_174877156.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17479.28 Da
• Isoelectric Point: 9.0439

>T207185 WP_039545981.1 NZ_CP077421:150175-150657 [Pectobacterium polaris]
MGITAPEPLSSGHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Nucleotide

• Length: 483 bp

>T207185 NZ_CP102929:c2440357-2440169 [Pseudomonas sp. CBS]
GTGCAAAGCAGGCTATTGATCAAAGAGCTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTGACGGGCAGCCATCATCTTTT
CACCCATCGCTATAACCCGTACACGATACCGGTGCCCCATCCCAAGAAGGACCTGCCGATGGGCACCGTGCGAAGTATCA
GGAAACGTGCCGGGCTGTTTTCGTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10609.08 Da
• Isoelectric Point: 4.4356

>AT207185 WP_039521400.1 NZ_CP077421:149906-150184 [Pectobacterium polaris]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITAERQAR
INDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT207185 NZ_CP102929:c2440147-2439743 [Pseudomonas sp. CBS]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTGTCGCCACCGGTATCCAGATCCCCGACATTCCAGGTGCGGT
GACCGCCGGGGACAGTTTTGAAGAGGCTTACAACGCGGCGGTCGAAGTGGCGCACATCATGCTGCAAGAGATCGCCGCAG
AAGGCCGGGCGATTCCGATGCCAACGTCGGTGGCGGCCCATCACGATAATGAGGATTACGCCGGCATGGGCTGGGGGATG
TTGGAGCTCGACATCTCGCCGTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTACGTGATCCAGCGTAT
CGACCGTTATGTGCGGGAGCACAAAGTGAAAAGCCGCTCGTCATTTCTGGCCGATGCGGCTTTGGAGAAGTTGGTGCGGT
TGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8QCC3