Detailed information of TA system TA207183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 311196..311731
• Replicon: plasmid unnamed2
• Accession: NZ_CP077418
• Organism: Rhodococcus qingshengii strain TG-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7I8BDH8
• Locus tag: KSE96_RS30600
• Protein ID: WP_042445814.1
• Coordinates: 311432..311731 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KSE96_RS30595
• Protein ID: WP_217018945.1
• Coordinates: 311196..311432 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSE96_RS30575 (KSE96_30575)	307396..308178	+	783	WP_029256883.1	ParA family protein	-
KSE96_RS33870	308343..308495	+	153	WP_007733689.1	hypothetical protein	-
KSE96_RS30585 (KSE96_30585)	309117..310091	+	975	WP_064688356.1	DUF2637 domain-containing protein	-
KSE96_RS30590 (KSE96_30590)	310201..310821	+	621	WP_257233646.1	TetR/AcrR family transcriptional regulator	-
KSE96_RS30595 (KSE96_30595)	311196..311432	+	237	WP_217018945.1	YlcI/YnfO family protein	Antitoxin
KSE96_RS30600 (KSE96_30600)	311432..311731	+	300	WP_042445814.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KSE96_RS30605 (KSE96_30605)	311760..313610	+	1851	WP_064688353.1	hypothetical protein	-
KSE96_RS30610 (KSE96_30610)	313610..314407	+	798	WP_070160714.1	hypothetical protein	-
KSE96_RS30615 (KSE96_30615)	314537..315607	-	1071	WP_064688351.1	PDDEXK nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..592026	592026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10441.94 Da
• Isoelectric Point: 4.3831

>T207183 WP_042445814.1 NZ_CP077418:311432-311731 [Rhodococcus qingshengii]
MRPIHEADLDTVRPVLVLTREVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T207183 NZ_CP102928:c817907-817629 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8652.67 Da
• Isoelectric Point: 6.4809

>AT207183 WP_217018945.1 NZ_CP077418:311196-311432 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVAFLDDEIREHRATSRASVVLRALERERRRQIAARDAEILANATPEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT207183 NZ_CP102928:c818188-817904 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: A0A7I8BDH8