Detailed information of TA system TA207181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 56804..57329
• Replicon: plasmid unnamed2
• Accession: NZ_CP077412
• Organism: Enterobacter asburiae strain FDAARGOS 1432

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: I6L62_RS22725
• Protein ID: WP_013023785.1
• Coordinates: 56804..57109 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: I6L62_RS22730
• Protein ID: WP_001568025.1
• Coordinates: 57111..57329 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L62_RS22695 (I6L62_22695)	51983..52417	+	435	WP_021312407.1	hypothetical protein	-
I6L62_RS22700 (I6L62_22700)	52707..53462	-	756	WP_001568031.1	replication initiation protein RepE	-
I6L62_RS22705 (I6L62_22705)	54093..54206	+	114	WP_014343462.1	hypothetical protein	-
I6L62_RS22710 (I6L62_22710)	54335..54592	+	258	WP_000764642.1	hypothetical protein	-
I6L62_RS22715 (I6L62_22715)	54650..55429	-	780	WP_001568028.1	site-specific integrase	-
I6L62_RS22720 (I6L62_22720)	55613..56617	-	1005	WP_001568027.1	hypothetical protein	-
I6L62_RS22725 (I6L62_22725)	56804..57109	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6L62_RS22730 (I6L62_22730)	57111..57329	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6L62_RS22735 (I6L62_22735)	57929..58159	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
I6L62_RS22740 (I6L62_22740)	58156..58572	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	-
I6L62_RS22745 (I6L62_22745)	58923..59891	-	969	WP_016151349.1	IS5 family transposase	-
I6L62_RS22750 (I6L62_22750)	60051..60314	+	264	WP_001295708.1	hypothetical protein	-
I6L62_RS22755 (I6L62_22755)	60508..60789	+	282	WP_023332908.1	helix-turn-helix domain-containing protein	-
I6L62_RS22760 (I6L62_22760)	60824..61393	+	570	WP_023332907.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118412	118412
	-	flank	IS/Tn	-	-	58923..59891	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T207181 WP_013023785.1 NZ_CP077412:c57109-56804 [Enterobacter asburiae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T207181 NZ_CP102928:810466-810768 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT207181 WP_001568025.1 NZ_CP077412:c57329-57111 [Enterobacter asburiae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT207181 NZ_CP102928:810221-810478 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8