Detailed information of TA system TA207179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4662645..4663221
• Replicon: chromosome
• Accession: NZ_CP077411
• Organism: Enterobacter asburiae strain FDAARGOS 1432

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A4R0G892
• Locus tag: I6L62_RS22180
• Protein ID: WP_010427219.1
• Coordinates: 4662645..4662932 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: I6L62_RS22185
• Protein ID: WP_214580031.1
• Coordinates: 4662919..4663221 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L62_RS22155 (I6L62_22155)	4657746..4658441	+	696	WP_217072132.1	winged helix-turn-helix domain-containing protein	-
I6L62_RS22160 (I6L62_22160)	4658591..4659061	+	471	WP_064673177.1	MarR family transcriptional regulator	-
I6L62_RS22165 (I6L62_22165)	4659058..4660125	+	1068	WP_217073247.1	HlyD family secretion protein	-
I6L62_RS22170 (I6L62_22170)	4660130..4661206	+	1077	WP_217072133.1	DUF2955 domain-containing protein	-
I6L62_RS22175 (I6L62_22175)	4661203..4662474	-	1272	WP_217072134.1	DUF445 domain-containing protein	-
I6L62_RS22180 (I6L62_22180)	4662645..4662932	+	288	WP_010427219.1	BrnT family toxin	Toxin
I6L62_RS22185 (I6L62_22185)	4662919..4663221	+	303	WP_214580031.1	BrnA antitoxin family protein	Antitoxin
I6L62_RS22190 (I6L62_22190)	4663252..4663890	-	639	WP_217072135.1	LysE family translocator	-
I6L62_RS22195 (I6L62_22195)	4663938..4664681	-	744	WP_217072136.1	AraC family transcriptional regulator	-
I6L62_RS22200 (I6L62_22200)	4664833..4666203	+	1371	WP_217072137.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
I6L62_RS22205 (I6L62_22205)	4666249..4666569	-	321	WP_033144551.1	hypothetical protein	-
I6L62_RS22210 (I6L62_22210)	4666569..4667114	-	546	WP_023310269.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11174.64 Da
• Isoelectric Point: 6.7609

>T207179 WP_010427219.1 NZ_CP077411:4662645-4662932 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T207179 NZ_CP102928:c806878-806381 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11421.07 Da
• Isoelectric Point: 10.1522

>AT207179 WP_214580031.1 NZ_CP077411:4662919-4663221 [Enterobacter asburiae]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT207179 NZ_CP102928:c807147-806875 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A4R0G892