Detailed information of TA system TA020717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2403889..2404862
• Replicon: chromosome
• Accession: NC_003909
• Organism: Bacillus cereus ATCC 10987

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BCE_RS12290
• Protein ID: WP_014893739.1
• Coordinates: 2403889..2404626 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A151UPU1
• Locus tag: BCE_RS12295
• Protein ID: WP_000588714.1
• Coordinates: 2404734..2404862 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCE_RS12270	2398971..2399753	+	783	WP_000381777.1	class I SAM-dependent methyltransferase	-
BCE_RS12275	2399931..2401616	-	1686	WP_011040340.1	alpha-keto acid decarboxylase family protein	-
BCE_RS12280	2401724..2402206	+	483	WP_000191907.1	MarR family winged helix-turn-helix transcriptional regulator	-
BCE_RS28545	2402313..2402477	+	165	Protein_2330	transposase	-
BCE_RS12285	2402814..2403551	+	738	WP_000594137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BCE_RS12290	2403889..2404626	+	738	WP_014893739.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BCE_RS12295	2404734..2404862	+	129	WP_000588714.1	hypothetical protein	Antitoxin
BCE_RS30040	2404935..2405111	+	177	WP_000852590.1	hypothetical protein	-
BCE_RS12305	2405130..2405519	-	390	WP_000714180.1	YxeA family protein	-
BCE_RS12310	2405736..2407205	+	1470	WP_000287562.1	beta-Ala-His dipeptidase	-
BCE_RS12315	2407406..2408012	+	607	Protein_2337	CatB-related O-acetyltransferase	-
BCE_RS12320	2408465..2409769	+	1305	WP_011040345.1	D-alanyl-D-alanine carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.07 Da
• Isoelectric Point: 8.2998

>T20717 WP_014893739.1 NC_003909:2403889-2404626 [Bacillus cereus ATCC 10987]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMVKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T20717 NC_003909:2403889-2404626 [Bacillus cereus ATCC 10987]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGGTAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCAAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAAAGCTTAAACGTATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGTTCTCAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGTAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAGTAG

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5024.48 Da
• Isoelectric Point: 4.0932

>AT20717 WP_000588714.1 NC_003909:2404734-2404862 [Bacillus cereus ATCC 10987]
VIKDYDNDFFKKDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT20717 NC_003909:2404734-2404862 [Bacillus cereus ATCC 10987]
GTGATAAAAGATTATGACAATGATTTTTTTAAAAAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAGCTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: A0A151UPU1