Detailed information of TA system TA207163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 70699..71462
• Replicon: plasmid unnamed1
• Accession: NZ_CP077408
• Organism: Enterobacter roggenkampii strain FDAARGOS 1430

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: I6L60_RS22765
• Protein ID: WP_069598995.1
• Coordinates: 70699..71193 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: I6L60_RS22770
• Protein ID: WP_069598994.1
• Coordinates: 71184..71462 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L60_RS22750 (I6L60_22750)	66308..69193	+	2886	WP_069598998.1	conjugal transfer mating-pair stabilization protein TraG	-
I6L60_RS22755 (I6L60_22755)	69209..69790	+	582	WP_069598997.1	protein traS	-
I6L60_RS22760 (I6L60_22760)	69935..70672	+	738	WP_069598996.1	conjugal transfer complement resistance protein TraT	-
I6L60_RS22765 (I6L60_22765)	70699..71193	-	495	WP_069598995.1	GNAT family N-acetyltransferase	Toxin
I6L60_RS22770 (I6L60_22770)	71184..71462	-	279	WP_069598994.1	DUF1778 domain-containing protein	Antitoxin
I6L60_RS22775 (I6L60_22775)	71602..73863	+	2262	WP_069598993.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..151584	151584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17987.76 Da
• Isoelectric Point: 9.3683

>T207163 WP_069598995.1 NZ_CP077408:c71193-70699 [Enterobacter roggenkampii]
MGISAPEPLKAGHNIADFCCSDQGLNEWLKKKAHKNHSTGISRVYVICAQNSQRVIGYYCLSTGSIQRKMAPGAYRRNSP
ESLPVIVLGRLAIDQSYCGKGLGAALLKDAIYRTENIALQAGVKALVVHALNEDVRSFYTRFGFDSSVVQPLTLLYPIKI
QTTE

Nucleotide

• Length: 495 bp

>T207163 NZ_CP102922:c100870-100559 [Klebsiella quasipneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10602.05 Da
• Isoelectric Point: 4.4513

>AT207163 WP_069598994.1 NZ_CP077408:c71462-71184 [Enterobacter roggenkampii]
MKTESRAAPINIRARESQRDLIDMAASLVSKSRTDFMLDVACREAQDILLDQRLFILDEEKYDAFVAELDAPITAERQAR
IDDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT207163 NZ_CP102922:c100562-100143 [Klebsiella quasipneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA