Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4241536..4242156 Replicon chromosome
Accession NZ_CP077407
Organism Enterobacter roggenkampii strain FDAARGOS 1430

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag I6L60_RS20040 Protein ID WP_008499287.1
Coordinates 4241536..4241754 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag I6L60_RS20045 Protein ID WP_008499288.1
Coordinates 4241782..4242156 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L60_RS20010 (I6L60_20010) 4237549..4237809 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
I6L60_RS20015 (I6L60_20015) 4237812..4237952 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I6L60_RS20020 (I6L60_20020) 4237949..4238659 - 711 WP_045352701.1 GNAT family protein -
I6L60_RS20025 (I6L60_20025) 4238761..4240221 + 1461 WP_063453152.1 PLP-dependent aminotransferase family protein -
I6L60_RS20030 (I6L60_20030) 4240193..4240660 - 468 WP_008499285.1 YlaC family protein -
I6L60_RS20035 (I6L60_20035) 4240778..4241329 - 552 WP_069597935.1 maltose O-acetyltransferase -
I6L60_RS20040 (I6L60_20040) 4241536..4241754 - 219 WP_008499287.1 HHA domain-containing protein Toxin
I6L60_RS20045 (I6L60_20045) 4241782..4242156 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
I6L60_RS20050 (I6L60_20050) 4242666..4245812 - 3147 WP_008499289.1 multidrug efflux RND transporter permease subunit AcrB -
I6L60_RS20055 (I6L60_20055) 4245835..4247028 - 1194 WP_008499290.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T207160 WP_008499287.1 NZ_CP077407:c4241754-4241536 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T207160 NZ_CP102921:3954420-3954638 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT207160 WP_008499288.1 NZ_CP077407:c4242156-4241782 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT207160 NZ_CP102921:3954019-3954393 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References