20716

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 274583..275225 Replicon chromosome
Accession NC_003909
Organism Bacillus cereus ATCC 10987

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCE_RS01485 Protein ID WP_000635963.1
Coordinates 274875..275225 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCE_RS01480 Protein ID WP_000004570.1
Coordinates 274583..274870 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCE_RS01455 269909..270871 + 963 WP_000961156.1 UV DNA damage repair endonuclease UvsE -
BCE_RS01460 270864..271436 - 573 WP_000907063.1 rhomboid family intramembrane serine protease -
BCE_RS01465 271529..271888 + 360 WP_000635037.1 holo-ACP synthase -
BCE_RS01470 272045..272995 + 951 WP_029141035.1 outer membrane lipoprotein carrier protein LolA -
BCE_RS01475 273114..274283 + 1170 WP_000390608.1 alanine racemase -
BCE_RS01480 274583..274870 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCE_RS01485 274875..275225 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCE_RS01490 275293..277461 + 2169 WP_000426205.1 RNA-binding transcriptional accessory protein -
BCE_RS01495 277519..277635 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCE_RS01500 277831..278289 + 459 WP_000344243.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T20716 WP_000635963.1 NC_003909:274875-275225 [Bacillus cereus ATCC 10987]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T20716 NC_003909:274875-275225 [Bacillus cereus ATCC 10987]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT20716 WP_000004570.1 NC_003909:274583-274870 [Bacillus cereus ATCC 10987]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT20716 NC_003909:274583-274870 [Bacillus cereus ATCC 10987]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References