Detailed information of TA system TA207159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3782626..3783202
• Replicon: chromosome
• Accession: NZ_CP077407
• Organism: Enterobacter roggenkampii strain FDAARGOS 1430

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A656ULU9
• Locus tag: I6L60_RS17900
• Protein ID: WP_025912099.1
• Coordinates: 3782626..3782913 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7D6YIG8
• Locus tag: I6L60_RS17905
• Protein ID: WP_025912101.1
• Coordinates: 3782900..3783202 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L60_RS17870 (I6L60_17870)	3777639..3777755	+	117	Protein_3486	amino acid-binding protein	-
I6L60_RS17875 (I6L60_17875)	3777748..3778443	+	696	WP_069597840.1	winged helix-turn-helix domain-containing protein	-
I6L60_RS17880 (I6L60_17880)	3778589..3779059	+	471	WP_008501325.1	MarR family transcriptional regulator	-
I6L60_RS17885 (I6L60_17885)	3779056..3780123	+	1068	WP_088728436.1	HlyD family secretion protein	-
I6L60_RS17890 (I6L60_17890)	3780113..3781189	+	1077	WP_069597841.1	DUF2955 domain-containing protein	-
I6L60_RS17895 (I6L60_17895)	3781186..3782457	-	1272	WP_069597842.1	DUF445 domain-containing protein	-
I6L60_RS17900 (I6L60_17900)	3782626..3782913	+	288	WP_025912099.1	BrnT family toxin	Toxin
I6L60_RS17905 (I6L60_17905)	3782900..3783202	+	303	WP_025912101.1	BrnA antitoxin family protein	Antitoxin
I6L60_RS17910 (I6L60_17910)	3783233..3783871	-	639	WP_069597843.1	LysE family translocator	-
I6L60_RS17915 (I6L60_17915)	3783919..3784662	-	744	WP_069597844.1	AraC family transcriptional regulator	-
I6L60_RS17920 (I6L60_17920)	3784814..3786184	+	1371	WP_069597845.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
I6L60_RS17925 (I6L60_17925)	3786230..3786550	-	321	WP_008501314.1	hypothetical protein	-
I6L60_RS17930 (I6L60_17930)	3786550..3787095	-	546	WP_008501313.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11235.68 Da
• Isoelectric Point: 7.4007

>T207159 WP_025912099.1 NZ_CP077407:3782626-3782913 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T207159 NZ_CP102921:c3834375-3834058 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCATGTGGACGTTAAAAAGGAGTTGCAGGCGCTTCCGGCTATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTTGGACAAGGGCTATTTGAAA
TTCGAGCGTTAGGTTCGGTACAGGGGCGGGGGATCTACGTTTTTCAGCAAAACAAAACGCTCTTTCTGCTACGTGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTTATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11413.02 Da
• Isoelectric Point: 10.0592

>AT207159 WP_025912101.1 NZ_CP077407:3782900-3783202 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT207159 NZ_CP102921:c3834065-3833778 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAGCACCGATTACGTACTATGCTGGCACAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTTGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCACCAGCCTGGATACCTTAACGCGA
TACGCGTTAGCGTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A656ULU9

Antitoxin

• AlphaFold DB: A0A7D6YIG8