Detailed information of TA system TA207158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3445616..3446213
• Replicon: chromosome
• Accession: NZ_CP077407
• Organism: Enterobacter roggenkampii strain FDAARGOS 1430

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: I6L60_RS16330
• Protein ID: WP_069597753.1
• Coordinates: 3445896..3446213 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: W7P674
• Locus tag: I6L60_RS16325
• Protein ID: WP_021242718.1
• Coordinates: 3445616..3445903 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L60_RS16320 (I6L60_16320)	3443988..3445619	+	1632	WP_008503411.1	Na/Pi cotransporter family protein	-
I6L60_RS16325 (I6L60_16325)	3445616..3445903	-	288	WP_021242718.1	putative addiction module antidote protein	Antitoxin
I6L60_RS16330 (I6L60_16330)	3445896..3446213	-	318	WP_069597753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L60_RS16335 (I6L60_16335)	3446411..3447283	+	873	WP_008503414.1	23S rRNA pseudouridine(2604) synthase RluF	-
I6L60_RS16340 (I6L60_16340)	3447284..3447556	-	273	WP_014830263.1	DUF3811 domain-containing protein	-
I6L60_RS16345 (I6L60_16345)	3447607..3448551	-	945	WP_023293551.1	ketopantoate/pantoate/pantothenate transporter PanS	-
I6L60_RS16350 (I6L60_16350)	3448657..3450231	-	1575	WP_069597754.1	RNA repair transcriptional activator RtcR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-91)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11835.67 Da
• Isoelectric Point: 9.6036

>T207158 WP_069597753.1 NZ_CP077407:c3446213-3445896 [Enterobacter roggenkampii]
MKEIVQTESFRRWEQNLKARRAKTIIASRLFRLANGLAGDVKPVGEGISELRIHFGPGYRIYFKDQGNYIIVLLCGGDKS
SQARDILMEKNAEQCIPMAGVNEHA

Nucleotide

• Length: 318 bp

>T207158 NZ_CP102921:c2568082-2566760 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACGCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGAATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCTATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATTTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGTTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGGGATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10156.57 Da
• Isoelectric Point: 6.4596

>AT207158 WP_021242718.1 NZ_CP077407:c3445903-3445616 [Enterobacter roggenkampii]
MHKLTPYDPANALVDDEEIAVFIADALETGDSTYIAKALGVVARAKGMSNISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGFGLTLKHAGD

Nucleotide

• Length: 288 bp

>AT207158 NZ_CP102921:c2568348-2568082 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: W7P674