Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 5548007..5548650 Replicon chromosome
Accession NZ_CP077405
Organism Citrobacter braakii strain FDAARGOS 1421

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag I6L51_RS26865 Protein ID WP_080861356.1
Coordinates 5548007..5548423 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A1V8NRE0
Locus tag I6L51_RS26870 Protein ID WP_064396317.1
Coordinates 5548420..5548650 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L51_RS26835 (I6L51_26835) 5543045..5543731 + 687 WP_217124989.1 lytic transglycosylase domain-containing protein -
I6L51_RS26840 (I6L51_26840) 5543733..5544302 + 570 WP_080861526.1 integrating conjugative element protein -
I6L51_RS26845 (I6L51_26845) 5544310..5544537 + 228 WP_143329773.1 hypothetical protein -
I6L51_RS26850 (I6L51_26850) 5544534..5546735 + 2202 WP_254170314.1 type IV conjugative transfer system coupling protein TraD -
I6L51_RS26855 (I6L51_26855) 5546735..5547481 + 747 WP_080861349.1 TIGR03747 family integrating conjugative element membrane protein -
I6L51_RS27305 5547726..5547902 + 177 Protein_5260 IS5/IS1182 family transposase -
I6L51_RS26865 (I6L51_26865) 5548007..5548423 - 417 WP_080861356.1 type II toxin-antitoxin system VapC family toxin Toxin
I6L51_RS26870 (I6L51_26870) 5548420..5548650 - 231 WP_064396317.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
I6L51_RS26875 (I6L51_26875) 5548817..5549124 + 308 Protein_5263 transposase -
I6L51_RS26880 (I6L51_26880) 5549534..5550265 + 732 WP_158083404.1 CZB domain-containing protein -
I6L51_RS26885 (I6L51_26885) 5550309..5550488 + 180 WP_143329765.1 hypothetical protein -
I6L51_RS26890 (I6L51_26890) 5550607..5550921 + 315 WP_080861364.1 RAQPRD family integrative conjugative element protein -
I6L51_RS26895 (I6L51_26895) 5550918..5551154 + 237 WP_080861366.1 TIGR03758 family integrating conjugative element protein -
I6L51_RS26900 (I6L51_26900) 5551205..5551546 + 342 WP_249025186.1 TIGR03745 family integrating conjugative element membrane protein -
I6L51_RS26905 (I6L51_26905) 5551562..5551942 + 381 WP_217124990.1 TIGR03750 family conjugal transfer protein -
I6L51_RS26910 (I6L51_26910) 5551942..5552598 + 657 WP_080861550.1 TIGR03746 family integrating conjugative element protein -
I6L51_RS26915 (I6L51_26915) 5552619..5553500 + 882 WP_254170325.1 TIGR03749 family integrating conjugative element protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5548874..5549134 260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-52)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15020.36 Da        Isoelectric Point: 6.7125

>T207146 WP_080861356.1 NZ_CP077405:c5548423-5548007 [Citrobacter braakii]
MIKTYMLDTNICSFIMREQPETVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRD
AVDATTEIKAALTAAGTSIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Download         Length: 417 bp

>T207146 NZ_CP102898:c3879084-3878767 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCACGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGGCCATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTGGGGCAGGGGCTATTCGAAA
TTCGGGCGTTAGGTTCGGTACAGGGGCGGGGGATTTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8685.75 Da        Isoelectric Point: 4.4563

>AT207146 WP_064396317.1 NZ_CP077405:c5548650-5548420 [Citrobacter braakii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLEKADSDFMAERGDIVSDEGRFDL

Download         Length: 231 bp

>AT207146 NZ_CP102898:c3878774-3878487 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACGGAACTGCTCAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACATCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1V8NRE0

References