Detailed information of TA system TA207144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4586251..4586867
• Replicon: chromosome
• Accession: NZ_CP077405
• Organism: Citrobacter braakii strain FDAARGOS 1421

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6L51_RS22225
• Protein ID: WP_016155182.1
• Coordinates: 4586251..4586625 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: I6L51_RS22230
• Protein ID: WP_016155181.1
• Coordinates: 4586625..4586867 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L51_RS22210 (I6L51_22210)	4583754..4584656	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
I6L51_RS22215 (I6L51_22215)	4584653..4585288	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
I6L51_RS22220 (I6L51_22220)	4585285..4586214	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
I6L51_RS22225 (I6L51_22225)	4586251..4586625	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6L51_RS22230 (I6L51_22230)	4586625..4586867	-	243	WP_016155181.1	CopG family transcriptional regulator	Antitoxin
I6L51_RS22235 (I6L51_22235)	4587072..4588001	+	930	WP_080859881.1	alpha/beta hydrolase	-
I6L51_RS22240 (I6L51_22240)	4588086..4588397	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	-
I6L51_RS22245 (I6L51_22245)	4588394..4588846	+	453	WP_080859878.1	helix-turn-helix domain-containing protein	-
I6L51_RS22250 (I6L51_22250)	4588864..4589805	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
I6L51_RS22255 (I6L51_22255)	4589850..4590287	-	438	WP_016157874.1	D-aminoacyl-tRNA deacylase	-
I6L51_RS22260 (I6L51_22260)	4590284..4591156	-	873	WP_019077938.1	virulence factor BrkB family protein	-
I6L51_RS22265 (I6L51_22265)	4591150..4591749	-	600	WP_016157873.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13678.84 Da
• Isoelectric Point: 8.5373

>T207144 WP_016155182.1 NZ_CP077405:c4586625-4586251 [Citrobacter braakii]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T207144 NZ_CP102898:2793786-2795108 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGCTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCCCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGAGCTGCTGGCCGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACTGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCACAAGAGGATCTGTGTCAGGCATTTGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCA
CAGGCCATCGAAAGCGCCCGCCGAACGCTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCGATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT207144 WP_016155181.1 NZ_CP077405:c4586867-4586625 [Citrobacter braakii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT207144 NZ_CP102898:2793520-2793786 [Klebsiella quasipneumoniae]
ATGCATTCCCCGCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCATCCGGACAAAACCACCTTGA
CCACCCTGTTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCCGAGACGGCGACGGCCCCC
GATAACACCACGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: R8WIS4