Detailed information of TA system TA207143

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4083744..4084447
• Replicon: chromosome
• Accession: NZ_CP077405
• Organism: Citrobacter braakii strain FDAARGOS 1421

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: I6L51_RS19900
• Protein ID: WP_080859436.1
• Coordinates: 4083744..4084085 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I6L51_RS19905
• Protein ID: WP_080859438.1
• Coordinates: 4084106..4084447 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L51_RS19870 (I6L51_19870)	4079500..4079862	+	363	WP_080859430.1	endoribonuclease SymE	-
I6L51_RS19875 (I6L51_19875)	4079920..4080404	-	485	Protein_3906	type VI secretion system tube protein Hcp	-
I6L51_RS19880 (I6L51_19880)	4080423..4080749	-	327	WP_080859432.1	DUF1493 family protein	-
I6L51_RS19885 (I6L51_19885)	4080743..4081192	-	450	WP_047414564.1	hypothetical protein	-
I6L51_RS19890 (I6L51_19890)	4081451..4082323	+	873	WP_080859434.1	HNH endonuclease	-
I6L51_RS19895 (I6L51_19895)	4082504..4083511	-	1008	WP_049017154.1	restriction endonuclease	-
I6L51_RS19900 (I6L51_19900)	4083744..4084085	-	342	WP_080859436.1	TA system toxin CbtA family protein	Toxin
I6L51_RS19905 (I6L51_19905)	4084106..4084447	-	342	WP_080859438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6L51_RS19910 (I6L51_19910)	4084458..4085000	-	543	WP_080859440.1	DNA repair protein RadC	-
I6L51_RS19915 (I6L51_19915)	4085013..4085456	-	444	WP_080859442.1	antirestriction protein	-
I6L51_RS19920 (I6L51_19920)	4085487..4086308	-	822	WP_080859444.1	DUF932 domain-containing protein	-
I6L51_RS19925 (I6L51_19925)	4086429..4086902	-	474	WP_049033293.1	hypothetical protein	-
I6L51_RS19930 (I6L51_19930)	4087003..4087467	-	465	WP_080859446.1	hypothetical protein	-
I6L51_RS19935 (I6L51_19935)	4087777..4088652	-	876	WP_080859448.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD	4079920..4093791	13871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12805.77 Da
• Isoelectric Point: 8.0324

>T207143 WP_080859436.1 NZ_CP077405:c4084085-4083744 [Citrobacter braakii]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T207143 NZ_CP102898:1950405-1950507 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12730.25 Da
• Isoelectric Point: 4.7424

>AT207143 WP_080859438.1 NZ_CP077405:c4084447-4084106 [Citrobacter braakii]
MKSTGSENDSFLQWGLNRNVTPCFGARLVQEGNQLHYLADQVSITGEFSNAESLKLDEVFPRFISQMESMLTTSEMNPRY
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT207143 NZ_CP102898:c1950511-1950364 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG