Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3464605..3465225 Replicon chromosome
Accession NZ_CP077405
Organism Citrobacter braakii strain FDAARGOS 1421

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I6L51_RS17035 Protein ID WP_002892050.1
Coordinates 3465007..3465225 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag I6L51_RS17030 Protein ID WP_019076175.1
Coordinates 3464605..3464979 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L51_RS17020 (I6L51_17020) 3459752..3460945 + 1194 WP_047416860.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6L51_RS17025 (I6L51_17025) 3460968..3464117 + 3150 WP_016152073.1 efflux RND transporter permease AcrB -
I6L51_RS17030 (I6L51_17030) 3464605..3464979 + 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
I6L51_RS17035 (I6L51_17035) 3465007..3465225 + 219 WP_002892050.1 HHA domain-containing protein Toxin
I6L51_RS17040 (I6L51_17040) 3465406..3465957 + 552 WP_046276082.1 maltose O-acetyltransferase -
I6L51_RS17045 (I6L51_17045) 3466074..3466544 + 471 WP_016152071.1 YlaC family protein -
I6L51_RS17050 (I6L51_17050) 3466623..3466763 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
I6L51_RS17055 (I6L51_17055) 3466765..3467025 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
I6L51_RS17060 (I6L51_17060) 3467214..3468767 + 1554 WP_080858963.1 EAL domain-containing protein -
I6L51_RS17065 (I6L51_17065) 3468819..3469172 - 354 WP_080858962.1 DUF1428 family protein -
I6L51_RS17070 (I6L51_17070) 3469237..3469866 - 630 WP_016155872.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207142 WP_002892050.1 NZ_CP077405:3465007-3465225 [Citrobacter braakii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207142 NZ_CP102898:779781-780191 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGTTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTAGTCTTTGACTGTG
TGCGTAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGTTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT207142 WP_019076175.1 NZ_CP077405:3464605-3464979 [Citrobacter braakii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT207142 NZ_CP102898:779534-779800 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References