Detailed information of TA system TA207131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxT-higA/Tad-HTH_37
• Location: 192085..192679
• Replicon: chromosome
• Accession: NZ_CP077405
• Organism: Citrobacter braakii strain FDAARGOS 1421

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: -
• Locus tag: I6L51_RS00890
• Protein ID: WP_080860924.1
• Coordinates: 192085..192405 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L51_RS00895
• Protein ID: WP_080860922.1
• Coordinates: 192395..192679 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L51_RS00870 (I6L51_00870)	187562..188052	+	491	Protein_173	reverse transcriptase domain-containing protein	-
I6L51_RS00875 (I6L51_00875)	188463..189872	+	1410	WP_080860928.1	group II intron reverse transcriptase/maturase	-
I6L51_RS00880 (I6L51_00880)	190767..191627	+	861	WP_080860926.1	alpha/beta hydrolase	-
I6L51_RS00885 (I6L51_00885)	191752..191943	+	192	Protein_176	IS110 family transposase	-
I6L51_RS00890 (I6L51_00890)	192085..192405	+	321	WP_080860924.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L51_RS00895 (I6L51_00895)	192395..192679	+	285	WP_080860922.1	helix-turn-helix transcriptional regulator	Antitoxin
I6L51_RS00910 (I6L51_00910)	193701..194495	-	795	WP_080860920.1	transcriptional regulator	-
I6L51_RS00915 (I6L51_00915)	194482..194985	-	504	WP_080860918.1	CaiF/GrlA family transcriptional regulator	-
I6L51_RS00920 (I6L51_00920)	195118..195864	-	747	WP_158083390.1	fimbrial protein	-
I6L51_RS00925 (I6L51_00925)	196301..197053	-	753	WP_080860914.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	63548..207136	143588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12262.25 Da
• Isoelectric Point: 9.2819

>T207131 WP_080860924.1 NZ_CP077405:192085-192405 [Citrobacter braakii]
MYELIFHPDAESEIYELEPVMQAKALKGLEKLEAKGPELRYPDTDIIDGGLFELRVGRKDITRTFFAYAKGRRIYILRTF
IKKTPKTPKAEIALAKSRWEELKDGS

Nucleotide

• Length: 321 bp

>T207131 NZ_CP102891:c5195412-5195029 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
AGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10212.81 Da
• Isoelectric Point: 9.6557

>AT207131 WP_080860922.1 NZ_CP077405:192395-192679 [Citrobacter braakii]
MAVKGIRFSEIKAKALANPEVKKAYEEETQEEELRAVLVEMKSKSGLTSTEIAARMGVSQPAVSRLERNVSSASLSTLQR
YAAACGMHIKLSLS

Nucleotide

• Length: 285 bp

>AT207131 NZ_CP102891:c5195654-5195412 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA