Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 3852933..3854556 Replicon chromosome
Accession NZ_CP077392
Organism Enterobacter hormaechei strain FDAARGOS 1435

Toxin (Protein)


Gene name hipA Uniprot ID A0A7D6U035
Locus tag I6L65_RS18485 Protein ID WP_020833804.1
Coordinates 3853231..3854556 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A7W3GWM9
Locus tag I6L65_RS18480 Protein ID WP_058656924.1
Coordinates 3852933..3853226 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L65_RS18455 (I6L65_18455) 3848099..3848278 + 180 WP_071685142.1 hypothetical protein -
I6L65_RS18460 (I6L65_18460) 3848444..3849100 + 657 WP_059509512.1 trypsin-like serine protease -
I6L65_RS18465 (I6L65_18465) 3849303..3849800 + 498 WP_000340139.1 membrane protein -
I6L65_RS18475 (I6L65_18475) 3851264..3852532 + 1269 WP_228288646.1 hypothetical protein -
I6L65_RS18480 (I6L65_18480) 3852933..3853226 + 294 WP_058656924.1 helix-turn-helix transcriptional regulator Antitoxin
I6L65_RS18485 (I6L65_18485) 3853231..3854556 + 1326 WP_020833804.1 type II toxin-antitoxin system HipA family toxin Toxin
I6L65_RS18490 (I6L65_18490) 3854617..3854823 + 207 WP_000134171.1 hypothetical protein -
I6L65_RS18495 (I6L65_18495) 3854921..3855487 + 567 WP_077909720.1 TnsD family Tn7-like transposition protein -
I6L65_RS18500 (I6L65_18500) 3855487..3855804 + 318 WP_058656922.1 hypothetical protein -
I6L65_RS18505 (I6L65_18505) 3855801..3856478 + 678 WP_080394746.1 IS21-like element helper ATPase IstB -
I6L65_RS18510 (I6L65_18510) 3856677..3857681 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
I6L65_RS18515 (I6L65_18515) 3857760..3858428 - 669 WP_058678057.1 glutathione S-transferase N-terminal domain-containing protein -
I6L65_RS18520 (I6L65_18520) 3858561..3859100 - 540 WP_004099036.1 peptide-methionine (S)-S-oxide reductase MsrA -
I6L65_RS18525 (I6L65_18525) 3859102..3859545 - 444 WP_004099035.1 peptide-methionine (R)-S-oxide reductase MsrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3849858..3850745 887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50307.23 Da        Isoelectric Point: 6.0252

>T207118 WP_020833804.1 NZ_CP077392:3853231-3854556 [Enterobacter hormaechei]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T207118 NZ_CP102889:c4617722-4617189 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11024.41 Da        Isoelectric Point: 9.9492

>AT207118 WP_058656924.1 NZ_CP077392:3852933-3853226 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKFNSPARQEDW

Download         Length: 294 bp

>AT207118 NZ_CP102889:c4617999-4617733 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6U035

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3GWM9

References