Detailed information of TA system TA020710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5043454..5043915
• Replicon: chromosome
• Accession: NC_003552
• Organism: Methanosarcina acetivorans C2A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q8TIM1
• Locus tag: MA_RS21525
• Protein ID: WP_011024016.1
• Coordinates: 5043673..5043915 (+)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: Q8TIM2
• Locus tag: MA_RS21520
• Protein ID: WP_011024015.1
• Coordinates: 5043454..5043672 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA_RS27650	5039302..5039517	+	216	WP_162013103.1	hypothetical protein	-
MA_RS21495 (MA4121)	5039654..5039875	+	222	WP_048065903.1	hypothetical protein	-
MA_RS21500 (MA4122)	5040083..5041369	-	1287	WP_011021010.1	transposase	-
MA_RS21505 (MA4123)	5041451..5042500	+	1050	Protein_4289	proteasome-activating nucleotidase	-
MA_RS21515 (MA4124)	5042936..5043307	-	372	WP_011024014.1	tyrosine-type recombinase/integrase	-
MA_RS21520 (MA4125)	5043454..5043672	+	219	WP_011024015.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MA_RS21525 (MA4126)	5043673..5043915	+	243	WP_011024016.1	type II toxin-antitoxin system HicA family toxin	Toxin
MA_RS21530 (MA4127)	5044148..5044756	-	609	WP_226990905.1	site-specific integrase	-
MA_RS29035	5044811..5045044	-	234	Protein_4294	phage integrase N-terminal SAM-like domain-containing protein	-
MA_RS21535 (MA4128)	5045217..5045450	-	234	WP_226990682.1	hypothetical protein	-
MA_RS21540 (MA4129)	5046162..5046599	+	438	WP_011024019.1	hypothetical protein	-
MA_RS29575	5046612..5046743	+	132	WP_282679178.1	hypothetical protein	-
MA_RS21545	5046757..5047002	+	246	WP_048065904.1	hypothetical protein	-
MA_RS21550 (MA4131)	5047389..5048819	+	1431	WP_011024021.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5030405..5054731	24326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-65)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-57)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9184.02 Da
• Isoelectric Point: 11.0632

>T20710 WP_011024016.1 NC_003552:5043673-5043915 [Methanosarcina acetivorans C2A]
MSKILPLPAKKVVKALENIGFEQIRQKGSHLFLRHPDGRTTIVPMHPTEKIGSGMINKIIKDAKITRNEWIELINRLILF

Nucleotide

• Length: 243 bp

>T20710 NC_003552:5043673-5043915 [Methanosarcina acetivorans C2A]
ATGAGTAAAATATTACCTCTTCCTGCAAAAAAAGTTGTAAAAGCACTTGAAAATATAGGTTTCGAGCAGATAAGGCAGAA
GGGTAGTCACTTATTTTTACGGCATCCAGATGGAAGGACCACTATAGTCCCAATGCACCCGACAGAAAAAATCGGAAGTG
GAATGATCAATAAAATAATAAAAGATGCAAAAATTACACGGAATGAATGGATTGAATTGATAAATAGACTTATTCTTTTC
TAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8287.35 Da
• Isoelectric Point: 4.0899

>AT20710 WP_011024015.1 NC_003552:5043454-5043672 [Methanosarcina acetivorans C2A]
MKEFTVLIEQDENGIYVASVPELPGCHTQAETLDELNRRIKEAIELYLEVEAEKEEESHLDFIGIQKVKVEV

Nucleotide

• Length: 219 bp

>AT20710 NC_003552:5043454-5043672 [Methanosarcina acetivorans C2A]
ATGAAAGAGTTTACCGTCTTAATCGAACAGGACGAAAATGGAATTTACGTGGCTTCAGTTCCAGAACTTCCAGGATGCCA
TACACAAGCTGAAACGCTTGACGAATTAAACAGAAGAATAAAAGAGGCAATAGAACTTTACCTTGAAGTTGAAGCCGAGA
AAGAGGAAGAAAGTCATCTTGATTTCATCGGAATACAGAAAGTTAAAGTTGAGGTTTGA

Structures

Toxin

• AlphaFold DB: Q8TIM1

Antitoxin

• AlphaFold DB: Q8TIM2