Detailed information of TA system TA207097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5298..5886
• Replicon: plasmid unnamed5
• Accession: NZ_CP077374
• Organism: Acinetobacter lwoffii strain FDAARGOS 1394

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6L24_RS16395
• Protein ID: WP_120366266.1
• Coordinates: 5599..5886 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6L24_RS16390
• Protein ID: WP_216986682.1
• Coordinates: 5298..5612 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L24_RS16365 (I6L24_16370)	453..1382	-	930	WP_127262067.1	replication initiation protein RepM	-
I6L24_RS16370 (I6L24_16375)	1957..3393	-	1437	WP_216986685.1	MobQ family relaxase	-
I6L24_RS16375 (I6L24_16380)	3603..3869	+	267	WP_216986687.1	mobilization protein	-
I6L24_RS16380 (I6L24_16385)	3887..4318	+	432	WP_216986686.1	hypothetical protein	-
I6L24_RS16385 (I6L24_16390)	4404..5225	-	822	WP_216986681.1	hypothetical protein	-
I6L24_RS16390 (I6L24_16395)	5298..5612	-	315	WP_216986682.1	BrnA antitoxin family protein	Antitoxin
I6L24_RS16395 (I6L24_16400)	5599..5886	-	288	WP_120366266.1	BrnT family toxin	Toxin
I6L24_RS16400 (I6L24_16405)	6197..6583	+	387	WP_216986683.1	hypothetical protein	-
I6L24_RS16405 (I6L24_16410)	6675..6956	-	282	WP_000985610.1	putative addiction module antidote protein	-
I6L24_RS16410 (I6L24_16415)	6957..7259	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6L24_RS16415 (I6L24_16420)	7432..8226	+	795	WP_216986684.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8723	8723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11291.66 Da
• Isoelectric Point: 5.2134

>T207097 WP_120366266.1 NZ_CP077374:c5886-5599 [Acinetobacter lwoffii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T207097 NZ_CP102883:866670-867080 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12267.84 Da
• Isoelectric Point: 7.2031

>AT207097 WP_216986682.1 NZ_CP077374:c5612-5298 [Acinetobacter lwoffii]
MSTVRYSRKELNEKFSEKQDAEIERLLTKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT207097 NZ_CP102883:866423-866689 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA