Detailed information of TA system TA207093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 45514..46102
• Replicon: plasmid unnamed1
• Accession: NZ_CP077370
• Organism: Acinetobacter lwoffii strain FDAARGOS 1394

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6L24_RS15515
• Protein ID: WP_216986650.1
• Coordinates: 45514..45801 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6L24_RS15520
• Protein ID: WP_216986651.1
• Coordinates: 45788..46102 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L24_RS15490 (I6L24_15495)	41817..41978	+	162	Protein_48	IS6 family transposase	-
I6L24_RS15495 (I6L24_15500)	42020..42322	-	303	WP_096902776.1	XRE family transcriptional regulator	-
I6L24_RS15500 (I6L24_15505)	42315..42671	-	357	WP_216986649.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6L24_RS15505 (I6L24_15510)	43307..44284	+	978	WP_004761028.1	TerC family protein	-
I6L24_RS15510 (I6L24_15515)	44682..45385	+	704	Protein_52	IS6-like element IS1008 family transposase	-
I6L24_RS15515 (I6L24_15520)	45514..45801	+	288	WP_216986650.1	BrnT family toxin	Toxin
I6L24_RS15520 (I6L24_15525)	45788..46102	+	315	WP_216986651.1	BrnA antitoxin family protein	Antitoxin
I6L24_RS15525 (I6L24_15530)	46711..48552	+	1842	WP_216986652.1	copper resistance system multicopper oxidase	-
I6L24_RS15530 (I6L24_15535)	48539..49528	+	990	WP_216986653.1	copper resistance protein B	-
I6L24_RS15535 (I6L24_15540)	49811..50464	+	654	WP_005086345.1	hypothetical protein	-
I6L24_RS15540 (I6L24_15545)	50489..50863	-	375	WP_086322809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..121296	121296
	-	inside	IScluster/Tn	-	-	41817..45385	3568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11311.69 Da
• Isoelectric Point: 5.2216

>T207093 WP_216986650.1 NZ_CP077370:45514-45801 [Acinetobacter lwoffii]
MEQYFEWDEAKNRKNQKKHDVSFETASLFFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T207093 NZ_CP102880:30633-30938 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12186.79 Da
• Isoelectric Point: 7.0149

>AT207093 WP_216986651.1 NZ_CP077370:45788-46102 [Acinetobacter lwoffii]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGTVPDDQLDLSDIPEVADWSNGVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT207093 NZ_CP102880:30413-30631 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA