Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2874214..2874798 Replicon chromosome
Accession NZ_CP077366
Organism Pantoea agglomerans strain FDAARGOS 1447

Toxin (Protein)


Gene name mazF Uniprot ID A0A379AH68
Locus tag I6L77_RS13215 Protein ID WP_062757507.1
Coordinates 2874463..2874798 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A379AIS5
Locus tag I6L77_RS13210 Protein ID WP_062757509.1
Coordinates 2874214..2874462 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L77_RS13190 (I6L77_13190) 2870447..2871058 - 612 WP_010245779.1 DUF2291 domain-containing protein -
I6L77_RS13195 (I6L77_13195) 2871104..2872039 - 936 WP_010670004.1 D-ribose ABC transporter substrate-binding protein -
I6L77_RS13200 (I6L77_13200) 2872095..2872892 - 798 WP_062757511.1 D-threitol dehydrogenase -
I6L77_RS13205 (I6L77_13205) 2873245..2874057 + 813 WP_010245789.1 DeoR/GlpR family DNA-binding transcription regulator -
I6L77_RS13210 (I6L77_13210) 2874214..2874462 + 249 WP_062757509.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6L77_RS13215 (I6L77_13215) 2874463..2874798 + 336 WP_062757507.1 endoribonuclease MazF Toxin
I6L77_RS13220 (I6L77_13220) 2875133..2875486 + 354 WP_073970644.1 DUF6404 family protein -
I6L77_RS13225 (I6L77_13225) 2876601..2877092 + 492 WP_010245819.1 hypothetical protein -
I6L77_RS13230 (I6L77_13230) 2877178..2878395 + 1218 WP_010669997.1 type IVB secretion system protein IcmH/DotU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-111)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12021.00 Da        Isoelectric Point: 8.7330

>T207083 WP_062757507.1 NZ_CP077366:2874463-2874798 [Pantoea agglomerans]
MVSRFVPDAGDIIWVDFDPTKGSEQAGHRPAIVLSPFAYNDKTGMCLCVPCTTLSKGYPFEVPISAEKESVALSDQVKSI
AWKVRGATKKGVVSANELQQIRAKIKVIIGL

Download         Length: 336 bp

>T207083 NZ_CP102877:c4627673-4627140 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9285.68 Da        Isoelectric Point: 4.2925

>AT207083 WP_062757509.1 NZ_CP077366:2874214-2874462 [Pantoea agglomerans]
MIHGKVKRWGNSPAIRIPATLMQALNLNIDDEIKIDLVEGKLIIEPVKQEVGYSLEELLEGVTEENLHDPVEWGEPVGKE
IW

Download         Length: 249 bp

>AT207083 NZ_CP102877:c4627950-4627684 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A379AH68

Antitoxin

Source ID Structure
AlphaFold DB A0A379AIS5

References