Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 2704385..2705014 Replicon chromosome
Accession NZ_CP077366
Organism Pantoea agglomerans strain FDAARGOS 1447

Toxin (Protein)


Gene name hicA Uniprot ID A0A379AGD8
Locus tag I6L77_RS12380 Protein ID WP_010245321.1
Coordinates 2704832..2705014 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A349IF99
Locus tag I6L77_RS12375 Protein ID WP_010245317.1
Coordinates 2704385..2704810 (-) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L77_RS12365 (I6L77_12365) 2699831..2700985 + 1155 WP_010245312.1 efflux RND transporter periplasmic adaptor subunit -
I6L77_RS12370 (I6L77_12370) 2700998..2704150 + 3153 WP_062757596.1 multidrug efflux RND transporter permease subunit OqxB -
I6L77_RS12375 (I6L77_12375) 2704385..2704810 - 426 WP_010245317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6L77_RS12380 (I6L77_12380) 2704832..2705014 - 183 WP_010245321.1 type II toxin-antitoxin system HicA family toxin Toxin
I6L77_RS12385 (I6L77_12385) 2705212..2705706 - 495 WP_010245324.1 Rrf2 family transcriptional regulator -
I6L77_RS12390 (I6L77_12390) 2705914..2707098 - 1185 WP_062757594.1 MFS transporter -
I6L77_RS12395 (I6L77_12395) 2707301..2708230 + 930 WP_033757557.1 DNA replication terminus site-binding protein -
I6L77_RS12400 (I6L77_12400) 2708274..2709671 - 1398 WP_010245332.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-82)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 7000.12 Da        Isoelectric Point: 11.0643

>T207082 WP_010245321.1 NZ_CP077366:c2705014-2704832 [Pantoea agglomerans]
MKYSEFRRWLIQQGAILTKHRSGSSHFKVFLGDRQSIFPDHGAKEMPEGTRRKILKDLGL

Download         Length: 183 bp

>T207082 NZ_CP102877:4025641-4025859 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15499.78 Da        Isoelectric Point: 4.8263

>AT207082 WP_010245317.1 NZ_CP077366:c2704810-2704385 [Pantoea agglomerans]
MFNYAVRLTHDAKTDTYEVSCRDLPLLHSVGDTVEEALAEAEDGLVTAISLEMDTRRPIPVASKPLEGEYIVSVPVLVAM
KAALYNAMIETGTRKVDLARQLGVNGPQVDRLLDVEHSSKVENVELALAQLGRKARIEIRT

Download         Length: 426 bp

>AT207082 NZ_CP102877:4025240-4025614 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A379AGD8

Antitoxin

Source ID Structure
AlphaFold DB A0A349IF99

References