Detailed information of TA system TA020708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 594596..595212
• Replicon: chromosome
• Accession: NC_003413
• Organism: Pyrococcus furiosus DSM 3638

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I6U642
• Locus tag: PF_RS02935
• Protein ID: WP_011011692.1
• Coordinates: 594811..595212 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I6UNL1
• Locus tag: PF_RS02930
• Protein ID: WP_011011691.1
• Coordinates: 594596..594823 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PF_RS11105 (PF0568)	590397..590813	-	417	WP_014835169.1	DUF1156 domain-containing protein	-
PF_RS02915 (PF0569)	590873..591298	+	426	WP_011011687.1	DUF365 domain-containing protein	-
PF_RS11110 (PF0570)	591279..591695	-	417	WP_011011688.1	hypothetical protein	-
PF_RS11115 (PF0571)	591722..592438	-	717	WP_223209002.1	hypothetical protein	-
PF_RS02925 (PF0572)	592552..594519	+	1968	WP_011011690.1	IGHMBP2 family helicase	-
PF_RS02930 (PF0573)	594596..594823	+	228	WP_011011691.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PF_RS02935 (PF0574)	594811..595212	+	402	WP_011011692.1	PIN domain-containing protein	Toxin
PF_RS11120	595359..595454	-	96	WP_237718490.1	hypothetical protein	-
PF_RS02940 (PF0575)	595671..596009	-	339	WP_011011693.1	PIN domain-containing protein	-
PF_RS02945 (PF0576)	595996..596188	-	193	Protein_588	DUF104 domain-containing protein	-
PF_RS02950 (PF0577)	596299..597150	+	852	WP_011011695.1	ABC transporter ATP-binding protein	-
PF_RS02955 (PF0578)	597147..597848	+	702	WP_011011696.1	hypothetical protein	-
PF_RS02960 (PF0579)	597845..598537	+	693	WP_011011697.1	permease	-
PF_RS02965 (PF0580)	598798..599799	+	1002	WP_011011698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (5-48)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15632.01 Da
• Isoelectric Point: 6.4890

>T20708 WP_011011692.1 NC_003413:594811-595212 [Pyrococcus furiosus DSM 3638]
MHAVIDTNVLIYDTFSDSEFHKESRSLLNSLDRWYIPSIVLQEYVWFFRSQGFSSREAKIMLSEYISDPRFRGLVEDHNV
ILRAIDILERENLSLSRFNDMIILVHAIEKGTLATFDQKLRKLARKLSVEILP

Nucleotide

• Length: 402 bp

>T20708 NC_003413:594811-595212 [Pyrococcus furiosus DSM 3638]
ATGCATGCAGTGATAGATACAAATGTTCTAATTTATGATACATTTAGCGATTCTGAGTTTCACAAAGAGTCTAGATCTCT
TTTGAATTCTCTTGATAGATGGTACATTCCTTCCATAGTACTTCAGGAGTATGTGTGGTTTTTTAGAAGTCAAGGATTTT
CAAGTAGAGAGGCCAAGATAATGCTCTCTGAATATATATCAGATCCAAGATTCAGAGGTCTTGTAGAAGACCACAATGTA
ATTCTTCGTGCCATTGACATTCTCGAAAGGGAAAACCTCTCTTTATCACGGTTTAATGATATGATAATTCTTGTTCATGC
AATAGAGAAGGGTACCCTAGCAACTTTTGATCAAAAACTCAGAAAACTTGCAAGGAAGTTGAGCGTTGAAATCTTGCCAT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8705.47 Da
• Isoelectric Point: 10.2059

>AT20708 WP_011011691.1 NC_003413:594596-594823 [Pyrococcus furiosus DSM 3638]
MPITKVTRNYQITIPAEIRKALGIKEGELLEVRLENGKIIIERLKKERKTLKLGKKLTLEEIEKAIEEGMKQCMQ

Nucleotide

• Length: 228 bp

>AT20708 NC_003413:594596-594823 [Pyrococcus furiosus DSM 3638]
ATGCCCATTACGAAGGTTACTAGAAACTATCAGATAACAATTCCTGCAGAGATTAGAAAGGCCCTTGGAATTAAAGAAGG
TGAACTGCTTGAAGTAAGGCTAGAAAATGGAAAAATAATTATAGAAAGACTCAAAAAAGAAAGAAAAACACTCAAGCTGG
GTAAAAAGTTAACACTCGAAGAAATTGAGAAAGCGATAGAGGAGGGAATGAAACAATGCATGCAGTGA

Structures

Toxin

• AlphaFold DB: I6U642

Antitoxin

• AlphaFold DB: I6UNL1