Detailed information of TA system TA207068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 86109..86802
• Replicon: plasmid unnamed2
• Accession: NZ_CP077333
• Organism: Lelliottia amnigena strain FDAARGOS 1446

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: I6L76_RS23475
• Protein ID: WP_153336993.1
• Coordinates: 86425..86802 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A543KTP2
• Locus tag: I6L76_RS23470
• Protein ID: WP_066690341.1
• Coordinates: 86109..86444 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L76_RS23450 (I6L76_23450)	82079..82546	+	468	WP_064645523.1	conjugal transfer protein TraD	-
I6L76_RS23455 (I6L76_23455)	83439..84692	-	1254	WP_009238019.1	MgtC/SapB family protein	-
I6L76_RS23460 (I6L76_23460)	84880..85146	+	267	WP_009277699.1	hypothetical protein	-
I6L76_RS23465 (I6L76_23465)	85515..86051	-	537	WP_153336992.1	transposase	-
I6L76_RS23470 (I6L76_23470)	86109..86444	-	336	WP_066690341.1	helix-turn-helix transcriptional regulator	Antitoxin
I6L76_RS23475 (I6L76_23475)	86425..86802	-	378	WP_153336993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L76_RS23480 (I6L76_23480)	86994..87577	+	584	Protein_100	recombinase family protein	-
I6L76_RS23485 (I6L76_23485)	87574..90606	+	3033	WP_216981927.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-4.3	-	1..123770	123770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.85 Da
• Isoelectric Point: 8.8675

>T207068 WP_153336993.1 NZ_CP077333:c86802-86425 [Lelliottia amnigena]
MADKEKPLEWIASSYRDLMALSPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEAMAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T207068 NZ_CP102869:c5208566-5208183 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12214.34 Da
• Isoelectric Point: 10.0996

>AT207068 WP_066690341.1 NZ_CP077333:c86444-86109 [Lelliottia amnigena]
MQKRIIEGVEAQRSSGNVFADLGLPDAEKLKIKTGLVIEIRKAMRALDLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPATEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT207068 NZ_CP102869:c5208808-5208566 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A543KTP2