Detailed information of TA system TA207067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 59711..60404
• Replicon: plasmid unnamed2
• Accession: NZ_CP077333
• Organism: Lelliottia amnigena strain FDAARGOS 1446

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: I6L76_RS23345
• Protein ID: WP_153336993.1
• Coordinates: 60027..60404 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A543KTP2
• Locus tag: I6L76_RS23340
• Protein ID: WP_066690341.1
• Coordinates: 59711..60046 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L76_RS23315 (I6L76_23315)	55990..56280	+	291	WP_254208132.1	hypothetical protein	-
I6L76_RS23320 (I6L76_23320)	56277..56756	+	480	WP_216982006.1	thermonuclease family protein	-
I6L76_RS23325 (I6L76_23325)	57041..58294	-	1254	WP_009238019.1	MgtC/SapB family protein	-
I6L76_RS23330 (I6L76_23330)	58482..58748	+	267	WP_009277699.1	hypothetical protein	-
I6L76_RS23335 (I6L76_23335)	59117..59653	-	537	WP_153336992.1	transposase	-
I6L76_RS23340 (I6L76_23340)	59711..60046	-	336	WP_066690341.1	helix-turn-helix transcriptional regulator	Antitoxin
I6L76_RS23345 (I6L76_23345)	60027..60404	-	378	WP_153336993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L76_RS23350 (I6L76_23350)	60596..61179	+	584	Protein_74	recombinase family protein	-
I6L76_RS23355 (I6L76_23355)	61176..64208	+	3033	WP_216981927.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-4.3	-	1..123770	123770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.85 Da
• Isoelectric Point: 8.8675

>T207067 WP_153336993.1 NZ_CP077333:c60404-60027 [Lelliottia amnigena]
MADKEKPLEWIASSYRDLMALSPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEAMAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T207067 NZ_CP102869:c4730715-4730431 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12214.34 Da
• Isoelectric Point: 10.0996

>AT207067 WP_066690341.1 NZ_CP077333:c60046-59711 [Lelliottia amnigena]
MQKRIIEGVEAQRSSGNVFADLGLPDAEKLKIKTGLVIEIRKAMRALDLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPATEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT207067 NZ_CP102869:c4730947-4730705 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A543KTP2