Detailed information of TA system TA207066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 39953..40646
• Replicon: plasmid unnamed1
• Accession: NZ_CP077332
• Organism: Lelliottia amnigena strain FDAARGOS 1446

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: I6L76_RS22375
• Protein ID: WP_153336993.1
• Coordinates: 40269..40646 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A543KTP2
• Locus tag: I6L76_RS22370
• Protein ID: WP_066690341.1
• Coordinates: 39953..40288 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L76_RS22340 (I6L76_22340)	36019..36555	+	537	WP_061549676.1	GntR family transcriptional regulator	-
I6L76_RS22345 (I6L76_22345)	36534..36674	-	141	Protein_40	IS3 family transposase	-
I6L76_RS24075	36794..36876	+	83	Protein_41	hypothetical protein	-
I6L76_RS22350 (I6L76_22350)	36912..37109	+	198	Protein_42	LacI family DNA-binding transcriptional regulator	-
I6L76_RS22355 (I6L76_22355)	37283..38536	-	1254	WP_009238019.1	MgtC/SapB family protein	-
I6L76_RS22360 (I6L76_22360)	38724..38990	+	267	WP_009277699.1	hypothetical protein	-
I6L76_RS22365 (I6L76_22365)	39359..39895	-	537	WP_153336992.1	transposase	-
I6L76_RS22370 (I6L76_22370)	39953..40288	-	336	WP_066690341.1	helix-turn-helix transcriptional regulator	Antitoxin
I6L76_RS22375 (I6L76_22375)	40269..40646	-	378	WP_153336993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L76_RS22380 (I6L76_22380)	40838..41421	+	584	Protein_48	recombinase family protein	-
I6L76_RS22385 (I6L76_22385)	41418..44450	+	3033	WP_216981927.1	Tn3 family transposase	-
I6L76_RS22390 (I6L76_22390)	44483..44710	+	228	Protein_50	DsbA family protein	-
I6L76_RS22395 (I6L76_22395)	44835..45362	+	528	WP_225986326.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..157312	157312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.85 Da
• Isoelectric Point: 8.8675

>T207066 WP_153336993.1 NZ_CP077332:c40646-40269 [Lelliottia amnigena]
MADKEKPLEWIASSYRDLMALSPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEAMAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T207066 NZ_CP102869:c4607552-4607019 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12214.34 Da
• Isoelectric Point: 10.0996

>AT207066 WP_066690341.1 NZ_CP077332:c40288-39953 [Lelliottia amnigena]
MQKRIIEGVEAQRSSGNVFADLGLPDAEKLKIKTGLVIEIRKAMRALDLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPATEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT207066 NZ_CP102869:c4607829-4607563 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A543KTP2