Detailed information of TA system TA207064

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4036479..4037158
• Replicon: chromosome
• Accession: NZ_CP077331
• Organism: Lelliottia amnigena strain FDAARGOS 1446

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: J7FU23
• Locus tag: I6L76_RS19500
• Protein ID: WP_015063033.1
• Coordinates: 4036817..4037158 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I6L76_RS19495
• Protein ID: WP_035893676.1
• Coordinates: 4036479..4036796 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L76_RS19475 (I6L76_19475)	4032607..4034802	+	2196	WP_216980920.1	DEAD/DEAH box helicase	-
I6L76_RS19480 (I6L76_19480)	4035258..4035737	+	480	WP_015063037.1	antirestriction protein	-
I6L76_RS19485 (I6L76_19485)	4035753..4036229	+	477	WP_015063036.1	RadC family protein	-
I6L76_RS19490 (I6L76_19490)	4036239..4036460	+	222	WP_015063035.1	DUF987 domain-containing protein	-
I6L76_RS19495 (I6L76_19495)	4036479..4036796	+	318	WP_035893676.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6L76_RS19500 (I6L76_19500)	4036817..4037158	+	342	WP_015063033.1	TA system toxin CbtA family protein	Toxin
I6L76_RS19510 (I6L76_19510)	4037521..4038027	+	507	WP_059177464.1	G/U mismatch-specific DNA glycosylase	-
I6L76_RS19515 (I6L76_19515)	4038149..4039987	-	1839	WP_041689526.1	RNA polymerase sigma factor RpoD	-
I6L76_RS19520 (I6L76_19520)	4040210..4041955	-	1746	WP_202693983.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD	4008015..4038027	30012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13051.09 Da
• Isoelectric Point: 9.9961

>T207064 WP_015063033.1 NZ_CP077331:4036817-4037158 [Lelliottia amnigena]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDTGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T207064 NZ_CP102869:c2645754-2644432 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTACTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTTACCGCCTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11837.51 Da
• Isoelectric Point: 6.6246

>AT207064 WP_035893676.1 NZ_CP077331:4036479-4036796 [Lelliottia amnigena]
MSNITLGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSEAECLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT207064 NZ_CP102869:c2646020-2645754 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: J7FU23