Detailed information of TA system TA020705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1044642..1045201
• Replicon: chromosome
• Accession: NC_003317
• Organism: Brucella melitensis bv. 1 str. 16M

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q8YH01
• Locus tag: BME_RS04995
• Protein ID: WP_004683741.1
• Coordinates: 1044642..1044887 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BME_RS05000
• Protein ID: WP_004683740.1
• Coordinates: 1044887..1045201 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME_RS04970	1040488..1041939	-	1452	WP_004685623.1	hypothetical protein	-
BME_RS04975	1042270..1043043	-	774	WP_002967634.1	hypothetical protein	-
BME_RS04990	1043871..1044509	-	639	Protein_981	tRNA-guanine transglycosylase	-
BME_RS04995	1044642..1044887	+	246	WP_004683741.1	BrnT family toxin	Toxin
BME_RS05000	1044887..1045201	+	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BME_RS05005	1045549..1045719	+	171	WP_004686810.1	YegP family protein	-
BME_RS05010	1046067..1046777	-	711	WP_004683739.1	porin family protein	-
BME_RS05015	1047012..1047599	-	588	WP_004683738.1	hypothetical protein	-
BME_RS05020	1047622..1047897	-	276	WP_002964092.1	hypothetical protein	-
BME_RS05025	1047894..1048124	-	231	WP_002964091.1	hypothetical protein	-
BME_RS05030	1048121..1048825	-	705	WP_005968944.1	hypothetical protein	-
BME_RS05035	1048875..1049078	+	204	WP_002964088.1	hypothetical protein	-
BME_RS05040	1049075..1049290	+	216	WP_005968947.1	hypothetical protein	-
BME_RS05045	1049293..1049496	+	204	WP_004683737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	1037374..1051268	13894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9196.83 Da
• Isoelectric Point: 10.3158

>T20705 WP_004683741.1 NC_003317:1044642-1044887 [Brucella melitensis bv. 1 str. 16M]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVFFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T20705 NC_003317:1044642-1044887 [Brucella melitensis bv. 1 str. 16M]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCTTTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT20705 WP_004683740.1 NC_003317:1044887-1045201 [Brucella melitensis bv. 1 str. 16M]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT20705 NC_003317:1044887-1045201 [Brucella melitensis bv. 1 str. 16M]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: Q8YH01

Antitoxin

• AlphaFold DB: C0RIV1