Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 76110..76907 Replicon plasmid unnamed2
Accession NZ_CP077310
Organism Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag I6L63_RS23540 Protein ID WP_006812498.1
Coordinates 76110..76634 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag I6L63_RS23545 Protein ID WP_006812497.1
Coordinates 76638..76907 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L63_RS23515 (I6L63_23515) 73781..74569 + 789 WP_032655902.1 receptor-recognizing protein -
I6L63_RS23520 (I6L63_23520) 74644..74967 + 324 WP_000064173.1 hypothetical protein -
I6L63_RS23525 (I6L63_23525) 74981..75673 + 693 WP_006812500.1 membrane protein -
I6L63_RS23530 (I6L63_23530) 75675..75926 + 252 WP_000147960.1 hypothetical protein -
I6L63_RS23535 (I6L63_23535) 75919..76083 + 165 WP_006812499.1 hypothetical protein -
I6L63_RS23540 (I6L63_23540) 76110..76634 - 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
I6L63_RS23545 (I6L63_23545) 76638..76907 - 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
I6L63_RS23550 (I6L63_23550) 77274..77939 + 666 WP_023315962.1 AAA family ATPase -
I6L63_RS23555 (I6L63_23555) 77945..78298 + 354 WP_160859104.1 hypothetical protein -
I6L63_RS23560 (I6L63_23560) 78340..79137 - 798 WP_006812584.1 DUF2971 domain-containing protein -
I6L63_RS23565 (I6L63_23565) 79401..80126 + 726 WP_006812583.1 hypothetical protein -
I6L63_RS23570 (I6L63_23570) 80187..81527 + 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..111400 111400


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T207037 WP_006812498.1 NZ_CP077310:c76634-76110 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T207037 NZ_CP102853:c2448866-2448690 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT207037 WP_006812497.1 NZ_CP077310:c76907-76638 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT207037 NZ_CP102853:c2448644-2448228 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References