Detailed information of TA system TA207036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 393954..394610
• Replicon: plasmid unnamed1
• Accession: NZ_CP077309
• Organism: Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6L63_RS22060
• Protein ID: WP_006812483.1
• Coordinates: 394218..394610 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5S3Q5
• Locus tag: I6L63_RS22055
• Protein ID: WP_006812484.1
• Coordinates: 393954..394214 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L63_RS22025 (I6L63_22025)	389155..389469	-	315	WP_006812490.1	PTS sugar transporter subunit IIB	-
I6L63_RS22030 (I6L63_22030)	389763..390713	+	951	WP_006812489.1	LacI family DNA-binding transcriptional regulator	-
I6L63_RS22035 (I6L63_22035)	390761..391057	-	297	WP_003861030.1	YicS family protein	-
I6L63_RS22040 (I6L63_22040)	391195..391614	+	420	WP_006812487.1	GNAT family N-acetyltransferase	-
I6L63_RS22045 (I6L63_22045)	391611..392258	-	648	WP_006812486.1	TetR/AcrR family transcriptional regulator	-
I6L63_RS22050 (I6L63_22050)	392337..393836	+	1500	WP_006812485.1	MDR family MFS transporter	-
I6L63_RS22055 (I6L63_22055)	393954..394214	+	261	WP_006812484.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6L63_RS22060 (I6L63_22060)	394218..394610	+	393	WP_006812483.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6L63_RS22065 (I6L63_22065)	394679..395152	-	474	WP_032674176.1	hypothetical protein	-
I6L63_RS22070 (I6L63_22070)	395534..396334	+	801	WP_032674175.1	lipoprotein NlpA	-
I6L63_RS22075 (I6L63_22075)	396368..397048	-	681	WP_000288876.1	EAL domain-containing protein	-
I6L63_RS22080 (I6L63_22080)	397124..397744	-	621	WP_006812480.1	LuxR C-terminal-related transcriptional regulator	-
I6L63_RS22085 (I6L63_22085)	398315..398950	+	636	WP_032674174.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	wza / rfaD / lpfA / lpfB / lpfC / lpfD / lpfE	1..606597	606597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14587.75 Da
• Isoelectric Point: 5.8985

>T207036 WP_006812483.1 NZ_CP077309:394218-394610 [Enterobacter hormaechei]
MLHMLDTNIVSHLVRQHPEVVNRYSQITPEKMCISSVTEAELLYGVAKKQNNKLHETIMEFLKTITICAWDSEAAATYGE
LRAAMEKKGKVMGDLDQLIAAHAISRGTTIVTNDHAFGMVQDLTVEDWTQ

Nucleotide

• Length: 393 bp

>T207036 NZ_CP102853:2359102-2360424 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGTCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10169.45 Da
• Isoelectric Point: 9.7289

>AT207036 WP_006812484.1 NZ_CP077309:393954-394214 [Enterobacter hormaechei]
MERTAKLFKKGRNQAVVLPAEFAFETESVYIRRDDEGNVVLTARSEKERHRDNFLRMLRQTHVPDTFLSKEERNQSYTTR
NPLEGL

Nucleotide

• Length: 261 bp

>AT207036 NZ_CP102853:2358836-2359102 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7T1QFB1