Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3457118..3457785 Replicon chromosome
Accession NZ_CP077308
Organism Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3MRP6
Locus tag I6L63_RS16840 Protein ID WP_001581456.1
Coordinates 3457456..3457785 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag I6L63_RS16835 Protein ID WP_000070396.1
Coordinates 3457118..3457435 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L63_RS16790 (I6L63_16790) 3452555..3453376 + 822 WP_000197389.1 DUF932 domain-containing protein -
I6L63_RS16795 (I6L63_16795) 3453593..3454294 + 702 WP_006812588.1 WYL domain-containing protein -
I6L63_RS16800 (I6L63_16800) 3454335..3454571 + 237 WP_001144031.1 protein YpjK -
I6L63_RS16805 (I6L63_16805) 3454571..3455014 + 444 WP_071850045.1 lipoprotein YafY -
I6L63_RS16810 (I6L63_16810) 3455038..3455505 + 468 WP_001581459.1 protein YkfB -
I6L63_RS16815 (I6L63_16815) 3455582..3455821 + 240 WP_000194654.1 DUF905 family protein -
I6L63_RS16820 (I6L63_16820) 3455919..3456377 + 459 WP_000211838.1 antirestriction protein -
I6L63_RS16825 (I6L63_16825) 3456393..3456869 + 477 WP_000811693.1 RadC family protein -
I6L63_RS16830 (I6L63_16830) 3456878..3457099 + 222 WP_000691994.1 DUF987 domain-containing protein -
I6L63_RS16835 (I6L63_16835) 3457118..3457435 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
I6L63_RS16840 (I6L63_16840) 3457456..3457785 + 330 WP_001581456.1 toxin Toxin
I6L63_RS16845 (I6L63_16845) 3458136..3458993 - 858 WP_001581455.1 DeoR/GlpR family DNA-binding transcription regulator -
I6L63_RS16850 (I6L63_16850) 3459124..3460074 - 951 WP_001581454.1 D-ribose ABC transporter substrate-binding protein -
I6L63_RS16855 (I6L63_16855) 3460163..3461224 - 1062 WP_001581452.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12456.52 Da        Isoelectric Point: 8.5011

>T207034 WP_001581456.1 NZ_CP077308:3457456-3457785 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Download         Length: 330 bp

>T207034 NZ_CP102853:1837876-1838130 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT207034 WP_000070396.1 NZ_CP077308:3457118-3457435 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT207034 NZ_CP102853:1837628-1837879 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MRP6

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References