Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2690657..2691454 Replicon chromosome
Accession NZ_CP077308
Organism Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)


Gene name KacT Uniprot ID F5RT91
Locus tag I6L63_RS13090 Protein ID WP_006809321.1
Coordinates 2690657..2691178 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag I6L63_RS13095 Protein ID WP_001303459.1
Coordinates 2691185..2691454 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L63_RS13060 (I6L63_13060) 2686255..2686944 + 690 WP_006809315.1 pyrimidine utilization protein B -
I6L63_RS13065 (I6L63_13065) 2686956..2687342 + 387 WP_006809316.1 pyrimidine utilization protein C -
I6L63_RS13070 (I6L63_13070) 2687350..2688150 + 801 WP_006809317.1 pyrimidine utilization protein D -
I6L63_RS13075 (I6L63_13075) 2688160..2688750 + 591 WP_006809318.1 malonic semialdehyde reductase -
I6L63_RS13080 (I6L63_13080) 2688760..2689254 + 495 WP_006809319.1 pyrimidine utilization flavin reductase protein F -
I6L63_RS13085 (I6L63_13085) 2689276..2690598 + 1323 WP_006809320.1 pyrimidine utilization transport protein G -
I6L63_RS13090 (I6L63_13090) 2690657..2691178 - 522 WP_006809321.1 GNAT family N-acetyltransferase Toxin
I6L63_RS13095 (I6L63_13095) 2691185..2691454 - 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
I6L63_RS13100 (I6L63_13100) 2691528..2692433 - 906 WP_006809322.1 DMT family transporter -
I6L63_RS13105 (I6L63_13105) 2692550..2692720 - 171 WP_001273664.1 general stress protein -
I6L63_RS13110 (I6L63_13110) 2693111..2693707 + 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
I6L63_RS13115 (I6L63_13115) 2693726..2693953 + 228 WP_006809324.1 YccJ family protein -
I6L63_RS13120 (I6L63_13120) 2693986..2695227 - 1242 WP_006809325.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
I6L63_RS13125 (I6L63_13125) 2695332..2695832 - 501 WP_032673548.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19494.28 Da        Isoelectric Point: 7.9346

>T207031 WP_006809321.1 NZ_CP077308:c2691178-2690657 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGNFDCGEPSLNAFLTDHLVRQHSGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQV

Download         Length: 522 bp

>T207031 NZ_CP102853:1000310-1000645 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT207031 WP_001303459.1 NZ_CP077308:c2691454-2691185 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT207031 NZ_CP102853:1000062-1000310 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGAGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1R089

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References