Detailed information of TA system TA207022

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 420937..421616
• Replicon: chromosome
• Accession: NZ_CP077308
• Organism: Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: I6L63_RS02085
• Protein ID: WP_006812012.1
• Coordinates: 421275..421616 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: I6L63_RS02080
• Protein ID: WP_006812013.1
• Coordinates: 420937..421254 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L63_RS02035 (I6L63_02035)	416374..417195	+	822	WP_000197389.1	DUF932 domain-containing protein	-
I6L63_RS02040 (I6L63_02040)	417412..418113	+	702	WP_006812588.1	WYL domain-containing protein	-
I6L63_RS02045 (I6L63_02045)	418154..418390	+	237	WP_001144031.1	protein YpjK	-
I6L63_RS02050 (I6L63_02050)	418390..418827	+	438	WP_016154551.1	lipoprotein YafY	-
I6L63_RS02055 (I6L63_02055)	418856..419323	+	468	WP_001547765.1	protein YkfB	-
I6L63_RS02060 (I6L63_02060)	419400..419639	+	240	WP_000194654.1	DUF905 family protein	-
I6L63_RS02065 (I6L63_02065)	419737..420195	+	459	WP_000211838.1	antirestriction protein	-
I6L63_RS02070 (I6L63_02070)	420211..420687	+	477	WP_071850078.1	RadC family protein	-
I6L63_RS02075 (I6L63_02075)	420696..420917	+	222	WP_001548171.1	DUF987 domain-containing protein	-
I6L63_RS02080 (I6L63_02080)	420937..421254	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6L63_RS02085 (I6L63_02085)	421275..421616	+	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
I6L63_RS02095 (I6L63_02095)	421978..422484	+	507	WP_006812011.1	G/U mismatch-specific DNA glycosylase	-
I6L63_RS02100 (I6L63_02100)	422579..424423	-	1845	WP_006812010.1	RNA polymerase sigma factor RpoD	-
I6L63_RS02105 (I6L63_02105)	424589..426334	-	1746	WP_006812009.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T207022 WP_006812012.1 NZ_CP077308:421275-421616 [Enterobacter hormaechei]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T207022 NZ_CP102850:c1141670-1141263 [Gordonia mangrovi]
GTGATCGTCGACACGAGCGCGCTGCTGGCGTACTTCGACGCCGCCGAACCGCAGCATGATGCCGTTGCCGAGTCGATCGA
GTCTGCGAGCGAGCCGCTGGTGGTGTCGCCGTATGTGGTCGCTGAGCTGGACTACCTGGTGTTGACCCGGCACGGGTCGC
GCGCCGAGCGATTGGTGCTCGCTGAGCTCGCCTCGGGCGCATGGGAACTTGCCGCGATGTCGCGTGATCGGCTCGCGGCG
GCGACAGCTGTTGTGGAGAAGTACGCCGACGTTCCGATCGGCATCGCCGATGCGTCGAACATCGTCCTGGCCGACGCTTA
CCAAACCAGGACGATTGCGACTCTCGATCGTCGGCACTTCGGTGTGTTGCGGCTCGGTGACGGATCAGCGCCGATCATCG
TGCCCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT207022 WP_006812013.1 NZ_CP077308:420937-421254 [Enterobacter hormaechei]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT207022 NZ_CP102850:c1141879-1141667 [Gordonia mangrovi]
ATGGCGAAGACCACCTTGTATCTCCCGGACGACCTGAAACGAGCCATCGAGCTCGAGGCGCGTCGCCGGGCGATCTCCGA
GGCAGAAGTCGTGCGGCAGGCGCTCCGTGAGGCATTGAGTGGCAACACCGTTCGTCCGCGGGGTGCGCTCTTTTCCGGGA
GTGAACCCATCGCCGATCGAGTAGACGAGCTACTCGACGGGTTCGGCGAGTGA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2