Detailed information of TA system TA207020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4721..5309
• Replicon: plasmid unnamed2
• Accession: NZ_CP077305
• Organism: Acinetobacter pittii strain FDAARGOS 1396

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6L26_RS18455
• Protein ID: WP_039890878.1
• Coordinates: 4721..5008 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6L26_RS18460
• Protein ID: WP_057095489.1
• Coordinates: 4995..5309 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L26_RS18440 (I6L26_18440)	2149..3078	+	930	WP_005237331.1	replication initiation protein RepM	-
I6L26_RS18445 (I6L26_18445)	3147..3692	+	546	WP_171054282.1	plasmid replication DNA-binding protein	-
I6L26_RS18450 (I6L26_18450)	3889..4023	-	135	Protein_2	cysteine hydrolase	-
I6L26_RS18455 (I6L26_18455)	4721..5008	+	288	WP_039890878.1	BrnT family toxin	Toxin
I6L26_RS18460 (I6L26_18460)	4995..5309	+	315	WP_057095489.1	BrnA antitoxin family protein	Antitoxin
I6L26_RS18465 (I6L26_18465)	5532..7670	+	2139	WP_057095490.1	ATP-binding domain-containing protein	-
I6L26_RS18470 (I6L26_18470)	7672..8343	+	672	WP_057095488.1	DUF2290 domain-containing protein	-
I6L26_RS18475 (I6L26_18475)	8464..9627	-	1164	WP_005237355.1	zinc-dependent alcohol dehydrogenase	-
I6L26_RS18480 (I6L26_18480)	9871..10203	-	333	WP_032049750.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11469	11469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2216

>T207020 WP_039890878.1 NZ_CP077305:4721-5008 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T207020 NZ_CP102850:c963926-963645 [Gordonia mangrovi]
ATGAGCGATTCGACGCCGCAACCCTACGACGTCGCCATCGCCTCACCCGCGCGCCGCGCCCTCTCGCGCCTTCCCGGACG
CGTTGCGCACGCCGTCATCGAGTTCATCTCCGGTCCGCTCGCCGAGAATCCGCGGCGGCTGAGCAAGCCGCTGCGCAACC
AGCTCGACGGCCTGCACAGTGCCCGTCGCGGCGACTATCGGATTCTCCTGCGGATCGATGATGACAACCGCACGGTGCTG
ATCGTCGACATCGACCATCGTGCGCATGTGTATCGCACATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12242.87 Da
• Isoelectric Point: 7.3173

>AT207020 WP_057095489.1 NZ_CP077305:4995-5309 [Acinetobacter pittii]
MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT207020 NZ_CP102850:c964198-963923 [Gordonia mangrovi]
ATGACGACCGTGCCGCTTGGCGAGGCCAAGGACAAGCTCTCCGCGCTGGTCGACAGCGCCGAGTCGACGCACGACATCAT
CACGATCACCAAACACGGTAAGCCGGCCGCGGTGCTCATGTCGGCCGACGACCTCGAATCGCTGCACGAGACGATCTACT
GGCTGTCGCGGCGCGGCACGGCCGAAGCGGTCGCCGCCGCCGACGACGAATCCGCGGCCGGAAAGACCATCAGTCTCGAC
GACCTGCGTGCCGAGCACGGTCTTCCCCCACGATGA