Detailed information of TA system TA020702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1813529..1814118
• Replicon: chromosome
• Accession: NC_003295
• Organism: Ralstonia solanacearum GMI1000

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: RS_RS08575
• Protein ID: WP_043876605.1
• Coordinates: 1813864..1814118 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q8XYR1
• Locus tag: RS_RS08570
• Protein ID: WP_011001638.1
• Coordinates: 1813529..1813867 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RS_RS08540	1809829..1810167	+	339	WP_011001632.1	phage tail protein	-
RS_RS08545	1810164..1810841	+	678	WP_011001633.1	phage tail protein	-
RS_RS08550	1810847..1811443	+	597	WP_011001634.1	tail fiber assembly protein	-
RS_RS08555	1811440..1812141	+	702	WP_011001635.1	phage minor tail protein L	-
RS_RS08560	1812143..1812853	+	711	WP_011001636.1	C40 family peptidase	-
RS_RS08565	1812857..1813459	+	603	WP_011001637.1	tail assembly protein	-
RS_RS08570	1813529..1813867	-	339	WP_011001638.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RS_RS08575	1813864..1814118	-	255	WP_043876605.1	type II toxin-antitoxin system HicA family toxin	Toxin
RS_RS08580	1814170..1817325	+	3156	WP_011001639.1	host specificity protein J	-
RS_RS08585	1817325..1817636	+	312	WP_043876606.1	hypothetical protein	-
RS_RS08590	1817662..1818318	+	657	WP_011001640.1	hypothetical protein	-
RS_RS08595	1818387..1818878	+	492	WP_011001641.1	glycoside hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1782376..1830338	47962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9488.05 Da
• Isoelectric Point: 10.3121

>T20702 WP_043876605.1 NC_003295:c1814118-1813864 [Ralstonia solanacearum GMI1000]
MKAKHQKTLELIFSRPTPAGVKWTDAVALMRELGAELEEREGSRVAVFLFGQVKVMHRPHPSPDMDKGAVASMRKWFEEN
GVKP

Nucleotide

• Length: 255 bp

>T20702 NC_003295:c1814118-1813864 [Ralstonia solanacearum GMI1000]
ATGAAAGCCAAACACCAGAAGACGCTGGAGCTGATCTTTTCCCGCCCTACGCCAGCGGGTGTTAAGTGGACCGATGCCGT
CGCACTCATGCGAGAACTGGGCGCGGAATTGGAGGAGCGCGAGGGTTCTCGCGTGGCCGTCTTCCTGTTTGGTCAAGTTA
AAGTGATGCATCGACCACATCCATCGCCCGATATGGACAAGGGCGCTGTGGCCTCGATGCGTAAGTGGTTCGAGGAAAAC
GGAGTCAAGCCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12007.63 Da
• Isoelectric Point: 5.1236

>AT20702 WP_011001638.1 NC_003295:c1813867-1813529 [Ralstonia solanacearum GMI1000]
MINVMNIGGHKAVIAYDPDIEMFRGEFVGLNGGADFYAADVPGLHREGELSLHVFLEECARRGVEPQKHFSGKFMLRVEG
KVHEAATIAAAAQGVSLNQWAAGVLEQAAEAA

Nucleotide

• Length: 339 bp

>AT20702 NC_003295:c1813867-1813529 [Ralstonia solanacearum GMI1000]
ATGATCAACGTCATGAACATCGGCGGCCACAAGGCCGTCATCGCCTACGACCCGGATATCGAAATGTTCCGTGGAGAGTT
TGTTGGCCTGAACGGTGGCGCCGATTTCTACGCCGCCGATGTACCCGGCCTGCACCGCGAAGGCGAGTTGTCGCTGCACG
TGTTTCTGGAAGAGTGCGCACGGCGTGGCGTTGAACCGCAAAAGCACTTTTCTGGCAAGTTCATGCTGCGAGTGGAGGGC
AAGGTGCACGAGGCCGCCACCATCGCCGCTGCCGCGCAAGGCGTGAGCCTTAACCAGTGGGCAGCCGGCGTGCTGGAGCA
AGCCGCCGAGGCGGCTTAA

Structures

Antitoxin

• AlphaFold DB: Q8XYR1