Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 1303937..1304535 Replicon chromosome
Accession NZ_CP077302
Organism Corynebacterium coyleae strain FDAARGOS 1425

Toxin (Protein)


Gene name PumA Uniprot ID A0A2I1V9Y7
Locus tag I6L55_RS06225 Protein ID WP_070482101.1
Coordinates 1304227..1304535 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A1F1YMH8
Locus tag I6L55_RS06220 Protein ID WP_070362126.1
Coordinates 1303937..1304230 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L55_RS06195 (I6L55_06195) 1300114..1300254 - 141 WP_232745367.1 hypothetical protein -
I6L55_RS06200 (I6L55_06200) 1300370..1301146 - 777 WP_208856578.1 ATP-binding protein -
I6L55_RS06205 (I6L55_06205) 1301463..1302702 - 1240 Protein_1197 IS256 family transposase -
I6L55_RS12170 1303458..1303940 + 483 WP_083308933.1 IS3 family transposase -
I6L55_RS06220 (I6L55_06220) 1303937..1304230 - 294 WP_070362126.1 putative addiction module antidote protein Antitoxin
I6L55_RS06225 (I6L55_06225) 1304227..1304535 - 309 WP_070482101.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L55_RS06230 (I6L55_06230) 1304842..1307010 - 2169 WP_224213107.1 hypothetical protein -
I6L55_RS06235 (I6L55_06235) 1307432..1308550 - 1119 Protein_1202 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1303937..1341883 37946
- inside IScluster/Tn - - 1301746..1308454 6708


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-96)

Antitoxin

Uncharacterized protein HI1420

(15-94)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11554.24 Da        Isoelectric Point: 9.4826

>T207012 WP_070482101.1 NZ_CP077302:c1304535-1304227 [Corynebacterium coyleae]
VEIISSSLFDAWLGELKDKHAQRRILYAIARCEAHGRMIGDIKPVGEKVSEMRFHFGPGYRVYYTRLGAITVFLLAGGDK
SSQDKDIRTAQKLAQQVREEQS

Download         Length: 309 bp

>T207012 NZ_CP102841:c4710884-4710600 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10578.05 Da        Isoelectric Point: 7.0095

>AT207012 WP_070362126.1 NZ_CP077302:c1304230-1303937 [Corynebacterium coyleae]
VKEVTFSAFDASKYLDSQEAVNDYLAIALEDGDTKTVQVVLRDIARAHGMSQLAKETQLNRESLYKSLSKDGNPSFAAIT
KIMKALGLKITLATQNA

Download         Length: 294 bp

>AT207012 NZ_CP102841:c4711116-4710874 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I1V9Y7

Antitoxin

Source ID Structure
AlphaFold DB A0A1F1YMH8

References