Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1548504..1550090 Replicon chromosome
Accession NC_003295
Organism Ralstonia solanacearum GMI1000

Toxin (Protein)


Gene name hipA Uniprot ID Q8XZF5
Locus tag RS_RS07280 Protein ID WP_011001395.1
Coordinates 1548504..1549841 (-) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag RS_RS07285 Protein ID WP_028853251.1
Coordinates 1549845..1550090 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RS_RS07255 1544149..1544889 + 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -
RS_RS07260 1544886..1545584 + 699 WP_019718855.1 protocatechuate 3,4-dioxygenase subunit alpha -
RS_RS07265 1545765..1546328 + 564 WP_080511083.1 RNA 2',3'-cyclic phosphodiesterase -
RS_RS07270 1546351..1547079 - 729 WP_011001393.1 DsbA family protein -
RS_RS07275 1547305..1548507 - 1203 WP_011001394.1 MFS transporter -
RS_RS07280 1548504..1549841 - 1338 WP_011001395.1 type II toxin-antitoxin system HipA family toxin Toxin
RS_RS07285 1549845..1550090 - 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
RS_RS07290 1550194..1551033 - 840 WP_011001397.1 oxaloacetate decarboxylase -
RS_RS07295 1551351..1551989 + 639 WP_011001398.1 7-carboxy-7-deazaguanine synthase -
RS_RS07300 1552057..1552533 + 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
RS_RS07305 1552553..1553104 + 552 WP_011001400.1 tRNA adenosine(34) deaminase TadA -
RS_RS07310 1553101..1554027 + 927 WP_011001401.1 muramoyltetrapeptide carboxypeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48742.83 Da        Isoelectric Point: 6.7232

>T20701 WP_011001395.1 NC_003295:c1549841-1548504 [Ralstonia solanacearum GMI1000]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEALTDSQVDTLLRNLTAMPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T20701 NC_003295:c1549841-1548504 [Ralstonia solanacearum GMI1000]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGCCTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACGGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGACCGCATCGACAGCGAGGCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCATGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCGCCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGCCGCTGGATGCCGGGCAACGCCTGGATCGCGCGCCTGCCGCAGGAAGACTTCTGCCAGGCTACAGGCACGCCCGC
GCACCGCAAATACGAAAACGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTGCTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTATGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGCTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCGAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT20701 WP_028853251.1 NC_003295:c1550090-1549845 [Ralstonia solanacearum GMI1000]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT20701 NC_003295:c1550090-1549845 [Ralstonia solanacearum GMI1000]
ATGGAGTCGCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTGCTGCGCATCCTGCACGCGCTCGGCGCGCAGCTGACGCTGCGCACGTCCGCGCCCGCCGCCGCCGCGCCCGCCGAC
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8XZF5

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References