Detailed information of TA system TA020700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 904468..905129
• Replicon: chromosome
• Accession: NC_003295
• Organism: Ralstonia solanacearum GMI1000

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Y122
• Locus tag: RS_RS04340
• Protein ID: WP_011000824.1
• Coordinates: 904468..904881 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8Y121
• Locus tag: RS_RS04345
• Protein ID: WP_011000825.1
• Coordinates: 904878..905129 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RS_RS04315	899833..900054	+	222	WP_011000818.1	hypothetical protein	-
RS_RS04320	900029..900673	+	645	WP_011000819.1	hypothetical protein	-
RS_RS04325	900716..901006	-	291	WP_011000820.1	H-NS histone family protein	-
RS_RS04330	901192..902658	-	1467	WP_043876564.1	type III secretion system YopJ family effector PopP2	-
RS_RS25365	903269..904396	+	1128	WP_080511079.1	DNA cytosine methyltransferase	-
RS_RS04340	904468..904881	-	414	WP_011000824.1	type II toxin-antitoxin system VapC family toxin	Toxin
RS_RS04345	904878..905129	-	252	WP_011000825.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RS_RS04350	905209..909309	+	4101	WP_011000826.1	tape measure protein	-
RS_RS04355	909315..909710	+	396	WP_011000827.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	861647..918732	57085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15591.91 Da
• Isoelectric Point: 5.7557

>T20700 WP_011000824.1 NC_003295:c904881-904468 [Ralstonia solanacearum GMI1000]
MSYLIDTNVLSELRRKAPDARVAAWMQDRPRQSLYLSVLTLGEIRKGIERLEDAVRRQNLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDIQLINPWEA

Nucleotide

• Length: 414 bp

>T20700 NC_003295:c904881-904468 [Ralstonia solanacearum GMI1000]
TTGAGTTACCTGATCGACACCAATGTCCTGTCCGAATTGCGTCGCAAAGCGCCCGATGCGCGCGTGGCGGCCTGGATGCA
GGACCGCCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAGATTCGCAAAGGTATCGAGCGGCTGGAGGACG
CGGTACGCCGCCAGAACCTGATTGACTGGCTCGAAGTGGAACTGCCGAACTACTTTCTCGGCCGATTGCTCGACATCGAT
GCACATACTGCGGACCGCTGGGGCCGGTTGATGTCCTCGGCTGGCCGCCCCCTGCCCGCCATTGACGGCCTGCTGGCCGC
GACCGCCCTGCAACACGACCTCACGCTGGTCACGCGCAACACCAAAGATTTCGCGGGGCTCGACATCCAGCTCATCAACC
CCTGGGAAGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9335.52 Da
• Isoelectric Point: 5.0774

>AT20700 WP_011000825.1 NC_003295:c905129-904878 [Ralstonia solanacearum GMI1000]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGSGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT20700 NC_003295:c905129-904878 [Ralstonia solanacearum GMI1000]
ATGCAAAGCTGGCAAATGCAGGCCGCCAAGGCGCGGTTTTCCGATGTCGTGAAGCGGGCAGCAGACGACGGCCCGCAGGA
AATCACCGTGCATGGCCGCCCGGTGGCGGTAGTGATCTCGCGCGCACTGTTCGATCGCTTGAGCGGCAGCGGTGAGTCAC
TGGTCAGCTTCATGCGCCAATCCCCGCTGGCCGATCAGGACGATGTCGTTTTTGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA

Structures

Toxin

• AlphaFold DB: Q8Y122

Antitoxin

• AlphaFold DB: Q8Y121