Detailed information of TA system TA206996

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1022785..1023439
• Replicon: chromosome
• Accession: NZ_CP077300
• Organism: Citrobacter braakii strain FDAARGOS 1422

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: I6L52_RS04890
• Protein ID: WP_151222347.1
• Coordinates: 1023032..1023439 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: I6L52_RS04885
• Protein ID: WP_016154349.1
• Coordinates: 1022785..1023051 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L52_RS04860 (1017997)	1017997..1019430	-	1434	WP_151222345.1	6-phospho-beta-glucosidase BglA	-
I6L52_RS04865 (1019552)	1019552..1020280	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
I6L52_RS04870 (1020333)	1020333..1020644	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
I6L52_RS04875 (1020808)	1020808..1021467	+	660	WP_151222346.1	hemolysin III family protein	-
I6L52_RS04880 (1021547)	1021547..1022527	-	981	WP_094168631.1	tRNA-modifying protein YgfZ	-
I6L52_RS04885 (1022785)	1022785..1023051	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
I6L52_RS04890 (1023032)	1023032..1023439	+	408	WP_151222347.1	protein YgfX	Toxin
I6L52_RS04895 (1023540)	1023540..1024061	-	522	WP_016154347.1	flavodoxin FldB	-
I6L52_RS04900 (1024175)	1024175..1025071	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
I6L52_RS04905 (1025095)	1025095..1025808	+	714	WP_047357820.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
I6L52_RS04910 (1025814)	1025814..1027547	+	1734	WP_049269301.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15892.70 Da
• Isoelectric Point: 11.5023

>T206996 WP_151222347.1 NZ_CP077300:1023032-1023439 [Citrobacter braakii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMIKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQARQG

Nucleotide

• Length: 408 bp

>T206996 NZ_CP102836:c5324687-5324304 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT206996 WP_016154349.1 NZ_CP077300:1022785-1023051 [Citrobacter braakii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT206996 NZ_CP102836:c5324929-5324687 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6