Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1113641..1114260 Replicon chromosome
Accession NZ_CP077293
Organism Klebsiella aerogenes strain FDAARGOS 1442

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag I6L72_RS05130 Protein ID WP_015367918.1
Coordinates 1113641..1113859 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag I6L72_RS05135 Protein ID WP_015367917.1
Coordinates 1113886..1114260 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L72_RS05100 (I6L72_05100) 1109714..1109974 + 261 WP_015367923.1 type B 50S ribosomal protein L31 -
I6L72_RS05105 (I6L72_05105) 1109977..1110117 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I6L72_RS05110 (I6L72_05110) 1110114..1110824 - 711 WP_015704607.1 GNAT family protein -
I6L72_RS05115 (I6L72_05115) 1110927..1112381 + 1455 WP_015704608.1 PLP-dependent aminotransferase family protein -
I6L72_RS05120 (I6L72_05120) 1112356..1112820 - 465 WP_015367920.1 YlaC family protein -
I6L72_RS25045 1112867..1112971 + 105 Protein_994 hypothetical protein -
I6L72_RS05125 (I6L72_05125) 1112946..1113509 - 564 WP_015704609.1 maltose O-acetyltransferase -
I6L72_RS05130 (I6L72_05130) 1113641..1113859 - 219 WP_015367918.1 HHA domain-containing protein Toxin
I6L72_RS05135 (I6L72_05135) 1113886..1114260 - 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
I6L72_RS05140 (I6L72_05140) 1114732..1117878 - 3147 WP_015704610.1 multidrug efflux RND transporter permease subunit AcrB -
I6L72_RS05145 (I6L72_05145) 1117901..1119100 - 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1104975..1114260 9285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T206983 WP_015367918.1 NZ_CP077293:c1113859-1113641 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T206983 NZ_CP102833:c5353999-5353616 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT206983 WP_015367917.1 NZ_CP077293:c1114260-1113886 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT206983 NZ_CP102833:c5354241-5353999 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References