Detailed information of TA system TA206980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 26821..27624
• Replicon: plasmid unnamed
• Accession: NZ_CP077292
• Organism: Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I6L53_RS23225
• Protein ID: WP_042325762.1
• Coordinates: 26821..27351 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: I6L53_RS23230
• Protein ID: WP_001275015.1
• Coordinates: 27355..27624 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L53_RS23225 (I6L53_23225)	26821..27351	-	531	WP_042325762.1	GNAT family N-acetyltransferase	Toxin
I6L53_RS23230 (I6L53_23230)	27355..27624	-	270	WP_001275015.1	DUF1778 domain-containing protein	Antitoxin
I6L53_RS23235 (I6L53_23235)	28484..29473	+	990	WP_084196684.1	RepB family plasmid replication initiator protein	-
I6L53_RS23240 (I6L53_23240)	29761..30501	-	741	WP_042325766.1	tyrosine-type recombinase/integrase	-
I6L53_RS23690	30658..30744	-	87	Protein_41	hypothetical protein	-
I6L53_RS23245 (I6L53_23245)	30865..31158	-	294	WP_042325767.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6L53_RS23250 (I6L53_23250)	31145..31411	-	267	WP_042325769.1	type II toxin-antitoxin system ParD family antitoxin	-
I6L53_RS23255 (I6L53_23255)	32104..32235	-	132	Protein_44	type II toxin-antitoxin system ParD family antitoxin	-
I6L53_RS23260 (I6L53_23260)	32326..32577	-	252	WP_042325774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88902	88902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20366.29 Da
• Isoelectric Point: 6.7494

>T206980 WP_042325762.1 NZ_CP077292:c27351-26821 [Citrobacter farmeri]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTAHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
RRVLYKNVPSVTLGRLALDKSIHHQGYGETLVTHAMKIVYQASQAVGIHGMFVEALNDNAKKFYLQLGFIQLKEENCNSL
FYPTKSIEELFEVRDE

Nucleotide

• Length: 531 bp

>T206980 NZ_CP077292:c27351-26821 [Citrobacter farmeri]
ATGGACGGTTTAAGGATTGAGATTTTTTCTGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACTGCTCATCTGAAGCGCCAGCACAACAGTAAGATTTTACGCGGCTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTTATGGGGTACTACACGTTGTCTGGCAGTTGCTTTGAGAAAACGCTGCTTCCCAGTAAGACACAACAA
AGGCGAGTGCTCTACAAAAACGTGCCGAGCGTGACTTTGGGTCGTCTGGCACTTGATAAAAGCATTCATCATCAGGGATA
TGGAGAAACACTTGTTACCCATGCTATGAAAATCGTGTATCAGGCCTCGCAAGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCAACTGGGTTTTATACAGTTAAAGGAAGAAAATTGTAATTCGCTT
TTCTACCCGACAAAATCGATTGAAGAATTGTTCGAAGTAAGAGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10257.69 Da
• Isoelectric Point: 5.7317

>AT206980 WP_001275015.1 NZ_CP077292:c27624-27355 [Citrobacter farmeri]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMEAITNPPAPNDRLKR
AAKRLQELE

Nucleotide

• Length: 270 bp

>AT206980 NZ_CP077292:c27624-27355 [Citrobacter farmeri]
ATGTCAGCACTGAAGAAGCAGCGCATAGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAGGCTGCAGCAAT
GACTAACCAGTCAATTTCACAGTTCATGGTCAGTACTGCTTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGAAGCAATCACTAATCCGCCGGCACCGAATGACCGACTGAAACGA
GCCGCTAAACGTCTCCAGGAACTGGAGTAA