Detailed information of TA system TA206979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 18446..18980
• Replicon: plasmid unnamed
• Accession: NZ_CP077292
• Organism: Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1V8P2
• Locus tag: I6L53_RS23190
• Protein ID: WP_000143805.1
• Coordinates: 18690..18980 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6L53_RS23185
• Protein ID: WP_042325750.1
• Coordinates: 18446..18700 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L53_RS23155 (I6L53_23155)	14581..14781	+	201	WP_042325742.1	hypothetical protein	-
I6L53_RS23160 (I6L53_23160)	14971..15822	+	852	WP_042325744.1	AAA family ATPase	-
I6L53_RS23165 (I6L53_23165)	15823..16116	+	294	WP_077958145.1	hypothetical protein	-
I6L53_RS23170 (I6L53_23170)	16171..16359	+	189	WP_042325747.1	hypothetical protein	-
I6L53_RS23175 (I6L53_23175)	17103..17498	+	396	WP_052425434.1	hypothetical protein	-
I6L53_RS23180 (I6L53_23180)	17584..18287	+	704	Protein_29	tyrosine-type recombinase/integrase	-
I6L53_RS23185 (I6L53_23185)	18446..18700	+	255	WP_042325750.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I6L53_RS23190 (I6L53_23190)	18690..18980	+	291	WP_000143805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L53_RS23195 (I6L53_23195)	19043..19306	+	264	WP_042325752.1	hypothetical protein	-
I6L53_RS23200 (I6L53_23200)	19387..19584	+	198	WP_042325754.1	hypothetical protein	-
I6L53_RS23205 (I6L53_23205)	19665..19835	+	171	WP_156970478.1	hypothetical protein	-
I6L53_RS23210 (I6L53_23210)	20340..20687	-	348	WP_042325758.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88902	88902
	-	flank	IS/Tn	-	-	20340..20687	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-73)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11463.41 Da
• Isoelectric Point: 10.2689

>T206979 WP_000143805.1 NZ_CP077292:18690-18980 [Citrobacter farmeri]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Nucleotide

• Length: 291 bp

>T206979 NZ_CP102833:4143580-4143798 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9708.04 Da
• Isoelectric Point: 4.4247

>AT206979 WP_042325750.1 NZ_CP077292:18446-18700 [Citrobacter farmeri]
MSTIQIRVDDELKKEAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSDDDEDADILAVVRERLKNPARRIRVN
VDDL

Nucleotide

• Length: 255 bp

>AT206979 NZ_CP102833:4143179-4143553 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A6P1V8P2