Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3447195..3447815 Replicon chromosome
Accession NZ_CP077291
Organism Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag I6L53_RS16200 Protein ID WP_001280991.1
Coordinates 3447597..3447815 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag I6L53_RS16195 Protein ID WP_042324455.1
Coordinates 3447195..3447569 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L53_RS16185 (I6L53_16185) 3442320..3443513 + 1194 WP_042324450.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6L53_RS16190 (I6L53_16190) 3443536..3446685 + 3150 WP_042324453.1 multidrug efflux RND transporter permease subunit AcrB -
I6L53_RS16195 (I6L53_16195) 3447195..3447569 + 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
I6L53_RS16200 (I6L53_16200) 3447597..3447815 + 219 WP_001280991.1 HHA domain-containing protein Toxin
I6L53_RS16205 (I6L53_16205) 3447993..3448544 + 552 WP_042324457.1 maltose O-acetyltransferase -
I6L53_RS16210 (I6L53_16210) 3448659..3449129 + 471 WP_042324459.1 YlaC family protein -
I6L53_RS16215 (I6L53_16215) 3449180..3449320 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I6L53_RS16220 (I6L53_16220) 3449543..3451093 + 1551 WP_042324461.1 EAL domain-containing protein -
I6L53_RS16225 (I6L53_16225) 3451133..3451486 - 354 WP_042324464.1 DUF1428 family protein -
I6L53_RS16230 (I6L53_16230) 3451569..3452165 - 597 WP_042324466.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T206975 WP_001280991.1 NZ_CP077291:3447597-3447815 [Citrobacter farmeri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206975 NZ_CP102833:813096-813506 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT206975 WP_042324455.1 NZ_CP077291:3447195-3447569 [Citrobacter farmeri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT206975 NZ_CP102833:812849-813115 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References