Detailed information of TA system TA206974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2371845..2372596
• Replicon: chromosome
• Accession: NZ_CP077291
• Organism: Citrobacter farmeri strain FDAARGOS 1423

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A8H9P063
• Locus tag: I6L53_RS11105
• Protein ID: WP_042319075.1
• Coordinates: 2372114..2372596 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A8H9TY90
• Locus tag: I6L53_RS11100
• Protein ID: WP_042319074.1
• Coordinates: 2371845..2372123 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L53_RS11085 (I6L53_11085)	2367844..2369205	-	1362	WP_042319070.1	MFS transporter	-
I6L53_RS11090 (I6L53_11090)	2369289..2370056	-	768	WP_042319071.1	SDR family oxidoreductase	-
I6L53_RS11095 (I6L53_11095)	2370394..2371647	+	1254	WP_042319072.1	enolase C-terminal domain-like protein	-
I6L53_RS11100 (I6L53_11100)	2371845..2372123	+	279	WP_042319074.1	DUF1778 domain-containing protein	Antitoxin
I6L53_RS11105 (I6L53_11105)	2372114..2372596	+	483	WP_042319075.1	GNAT family N-acetyltransferase	Toxin
I6L53_RS11110 (I6L53_11110)	2372622..2372987	-	366	WP_042319077.1	hypothetical protein	-
I6L53_RS11115 (I6L53_11115)	2373079..2373837	+	759	WP_042320028.1	trans-aconitate 2-methyltransferase	-
I6L53_RS11120 (I6L53_11120)	2373840..2374316	-	477	WP_042320030.1	CaiF/GrlA family transcriptional regulator	-
I6L53_RS11125 (I6L53_11125)	2374580..2377369	-	2790	WP_052425379.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2364826..2372987	8161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17458.34 Da
• Isoelectric Point: 9.2027

>T206974 WP_042319075.1 NZ_CP077291:2372114-2372596 [Citrobacter farmeri]
MGIRAPEPLTPGHIVAEFCCSDPGLNEWLRKKALKNHSTGISRVYVICAEETNRVIGYYCLSTGSVQRNTVPGSYRRNAP
ESLPVVVLGRLAVDQSCAGKGLGVALLRDAIFRTENIALQVGVRALIVHALNDDVKSFYMKFAFAPSSINPLTLLYPIKV

Nucleotide

• Length: 483 bp

>T206974 NZ_CP102833:327277-327645 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10562.05 Da
• Isoelectric Point: 4.6722

>AT206974 WP_042319074.1 NZ_CP077291:2371845-2372123 [Citrobacter farmeri]
MKPEAREAPINIRAKTSQRDLIDMAASLVSKSRTDFVLEAACREAQDILLDQRLFILDDKQYDAFLAELDAPITPERQAR
INDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT206974 NZ_CP102833:327059-327280 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA